Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults
10.5051/jpis.2018.48.4.261
- Author:
Heeyoung CHOI
1
;
Eunhye KIM
;
Jihoon KANG
;
Hyun Joo KIM
;
Ju Youn LEE
;
Jeomil CHOI
;
Ji Young JOO
Author Information
1. Department of Periodontology, Institute of Translational Dental Sciences, Pusan National University School of Dentistry, Yangsan, Korea. joojy@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Bacterial load;
Chronic periodontitis;
Real-time polymerase chain reaction;
Saliva;
Young adult
- MeSH:
Aggregatibacter actinomycetemcomitans;
Bacteria;
Bacterial Load;
Campylobacter rectus;
Chronic Periodontitis;
DNA;
Eikenella corrodens;
Female;
Forsythia;
Fusobacterium nucleatum;
Healthy Volunteers;
Humans;
Male;
Peptostreptococcus;
Periodontal Diseases;
Porphyromonas gingivalis;
Prevalence;
Prevotella intermedia;
Real-Time Polymerase Chain Reaction;
Saliva;
Treponema denticola;
Young Adult
- From:Journal of Periodontal & Implant Science
2018;48(4):261-271
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Few studies have examined periodontal pathogens from saliva samples in periodontally healthy young adults. The purposes of this study were to determine the prevalence of periodontopathic bacteria and to quantify periodontal pathogens in saliva samples using real-time polymerase chain reaction (PCR) assays in periodontally healthy Korean young adults under 35 years of age. METHODS: Nine major periodontal pathogens were analyzed by real-time PCR in saliva from 94 periodontally healthy young adults. Quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Campylobacter rectus, Peptostreptococcus anaerobius, and Eikenella corrodens was performed by DNA copy number measurement. RESULTS: F. nucleatum and E. corrodens were detected in all subjects; the numbers of positive samples were 87 (92.6%), 91 (96.8%), and 90 (95.7%) for P. gingivalis, P. anaerobius, and C. rectus, respectively. Other pathogens were also detected in periodontally healthy subjects. Analysis of DNA copy numbers revealed that the most abundant periodontal pathogen was F. nucleatum, which was significantly more prevalent than all other bacteria (P < 0.001), followed by P. anaerobius, P. gingivalis, E. corrodens, C. rectus, and T. denticola. There was no significant difference in the prevalence of each bacterium between men and women. The DNA copy number of total bacteria was significantly higher in men than in women. CONCLUSIONS: Major periodontal pathogens were prevalent in the saliva of periodontally healthy Korean young adults. Therefore, we suggest that the development of periodontal disease should not be overlooked in periodontally healthy young people, as it can arise due to periodontal pathogen imbalance and host susceptibility.
