An in vitro model of Fusobacterium nucleatum and Porphyromonas gingivalis in single- and dual-species biofilms
10.5051/jpis.2018.48.1.12
- Author:
Lívia Jacovassi TAVARES
1
;
Marlise Inêz KLEIN
;
Beatriz Helena Dias PANARIELLO
;
Erica DORIGATTI DE AVILA
;
Ana Cláudia PAVARINA
Author Information
1. Department of Dental Materials and Prosthodontics, São Paulo State University
- Publication Type:Original Article
- Keywords:
Bacterial adhesion;
Biofilms;
Fusobacterium nucleatum;
Porphyromonas gingivalis
- MeSH:
Bacterial Adhesion;
Biofilms;
Biomass;
Fusobacterium nucleatum;
Fusobacterium;
Gentian Violet;
In Vitro Techniques;
Microscopy, Electron, Scanning;
Polystyrenes;
Porphyromonas gingivalis;
Porphyromonas
- From:Journal of Periodontal & Implant Science
2018;48(1):12-21
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. METHODS: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. RESULTS: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. CONCLUSIONS: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.
