Mitigation effects of red Platycodon grandiflorum extract on lipopolysaccharide-induced inflammation in splenocytes isolated from mice
10.4163/jnh.2019.52.3.243
- Author:
Eun Jung PARK
1
;
You Suk LEE
;
Hyun Cheol JEONG
;
Sung Hyen LEE
;
Hae Jeung LEE
Author Information
1. Department of Food and Nutrition, Gachon University, Seongnam, Gyeonggi 13120, Korea. skysea@gachon.ac.kr
- Publication Type:Original Article
- Keywords:
Platycodon grandiflorum;
anti-inflammatory;
splenocytes
- MeSH:
Animals;
Cell Count;
Cell Survival;
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Inflammation;
Interleukin-10;
Interleukin-6;
Mice;
Nitric Oxide;
Platycodon;
Saponins
- From:Journal of Nutrition and Health
2019;52(3):243-249
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Platycodon grandiflorum (PG) is known to have effective antimicrobial and anticancer activity. The main bioactive components of PG are saponins, and these could contribute to anti-inflammatory activity. However, little is known about the anti-inflammatory effect of PG. In this study, we aim to assess the anti-inflammatory response to Red PG Extract (RPGE) in splenocytes under ex vivo conditions. METHODS: The cell viability of isolated splenocytes taken from mice was analyzed by performing a Cell Counting Kit-8 assay. The productions of nitric oxide (NO) and cytokines (specifically interleukin-6 (IL-6) and interleukin-10 (IL-10)) were measured utilizing Griess reagent and ELISA, respectively. RESULTS: We found that co-treatment with RPGE and Lipopolysaccharide (LPS) decreased isolated splenocyte proliferation as compared with that of the LPS-stimulated control. We also observed that RPGE markedly suppressed NO synthesis and IL-6 production that was induced by LPS. There were no significant differences of IL-10 production between co-treatment with RPGE plus LPS and treatment with LPS alone. CONCLUSION: When taken together, our data has shown that RPGE mitigates LPS-induced inflammation in splenocytes isolated from mice. Further research is surely needed to confirm the anti-inflammation effects of RPGE in an in vivo model.
