Degranulated Eosinophils Contain More Fine Nerve Fibers in the Duodenal Mucosa of Patients With Functional Dyspepsia
- Author:
Min Jin LEE
1
;
Hye Kyung JUNG
;
Ko Eun LEE
;
Yeung Chul MUN
;
Sanghui PARK
Author Information
- Publication Type:Original Article
- Keywords: Duodenum; Dyspepsia; Eosinophils; Inflammation; Peripheral nervous system
- MeSH: Biopsy; Case-Control Studies; Duodenitis; Duodenum; Dyspepsia; Eosine Yellowish-(YS); Eosinophils; Female; Hematoxylin; Humans; Hypersensitivity; Inflammation; Mucous Membrane; Nerve Fibers; Peripheral Nervous System; Prospective Studies; Stomach; Tertiary Care Centers
- From:Journal of Neurogastroenterology and Motility 2019;25(2):212-221
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS: Functional dyspepsia (FD) is characterized as chronic recurrent upper gastrointestinal symptoms in the absence of any organic disorder. We hypothesized that duodenal low-grade inflammation activates superficial afferent nerve sprouting, thereby contributing to hypersensitivity in patients with FD. METHODS: A prospective case-control study was conducted in a tertiary referral center. FD was defined using the Rome III criteria. Standardized endoscopic biopsies were performed in the stomach and duodenum. Hematoxylin and eosin staining and immunohistochemical staining for major basic proteins were performed to detect granulated eosinophil-derived granules, and S-100 staining was performed to detect fine nerve fibers. RESULTS: A total of 51 patients with FD (82% female; mean age 35.8 ± 13.4 years) and 35 controls were enrolled. Activated eosinophil counts in the duodenum were significantly higher in patients with FD than in controls (41.4% vs 17.1%, P = 0.005). Microscopic duodenitis was more frequently detected in patients with FD than in controls. Fine nerve fibers were more abundant in patients with FD than in controls (45.1% vs 11.4%, P = 0.029). The abundance of fine nerve fibers highly correlated with the degree of activated eosinophils. CONCLUSION: Duodenal low-grade inflammation, such as mucosal eosinophilic accumulation with degranulation, promoted mucosal enteric nerve fiber density and sprouting in patients with FD.
