Comparison of Red Blood Cell, White Blood Cell and Differential Counts between UF-5000 System and Manual Method
10.15263/jlmqa.2019.41.3.172
- Author:
Mo Sae KOO
1
;
Jinsook LIM
;
Seon Young KIM
;
Sun Hoe KOO
;
Gye Cheol KWON
Author Information
1. Department of Laboratory Medicine, Chungnam National University College of Medicine, Daejeon, Korea. kckwon00@naver.com
- Publication Type:Original Article
- Keywords:
Sysmex UF-5000;
Automation;
Laboratory;
Body fluids
- MeSH:
Automation;
Body Fluids;
Cell Count;
Cerebrospinal Fluid;
Erythrocytes;
Leukocytes;
Methods;
Microscopy;
Treatment Outcome
- From:Journal of Laboratory Medicine and Quality Assurance
2019;41(3):172-178
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Analysis of body fluids provides important information for assessing various medical conditions. We aimed to validate the analytical and diagnostic performance of the Sysmex UF-5000 (Sysmex, Japan) system for the analysis of different body fluids. METHODS: Eighty body fluid samples were analyzed using the UF-5000 system in the body fluid mode and light microscopy. Body fluids included ascitic, pleural, and cerebrospinal fluid (CSF), as well as other fluid samples. RESULTS: A comparison between the UF-5000 system and manual counting demonstrated good correlations with regard to red (r=0.6555) and white blood cell (r=0.9666) counts. The UF-5000 system also demonstrated good performance for differential cell counting (r=0.9028). CSF particularly showed a good correlation. CONCLUSIONS: The use of the UF-5000 system for cell counting and differential analysis of body fluid samples might be an effective and automated alternative to chamber counting in laboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness.
