Bone Regeneration Using Block-type Deproteinized Porcine Bone Mineral with Collagen Membrane Using 3,4-Dihydroxyphenylalanine as Bone Adhesive
10.5856/JKDS.2018.11.2.43
- Author:
Joo Hyun KANG
1
;
Hyoung Chul PAE
;
Jae Kook CHA
;
Jung Seok LEE
;
Jeong Won PAIK
;
Ui Won JUNG
;
Seong Ho CHOI
Author Information
1. Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea. shchoi726@yuhs.ac
- Publication Type:Original Article
- Keywords:
Adhesive;
Bone regeneration;
Dihydroxyphenyalanine
- MeSH:
Adhesiveness;
Adhesives;
Animals;
Bone Regeneration;
Cell Survival;
Collagen;
Cyanoacrylates;
Dihydroxyphenylalanine;
Membranes;
Miners;
Osteogenesis;
Skull;
X-Ray Microtomography
- From:Journal of Korean Dental Science
2018;11(2):43-56
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The purpose of this study was to assess the adhesiveness and cytotoxicity of 3, 4-dihydroxyphenylalanine (DOPA), and to evaluate the role of collagen membrane with DOPA in the guided bone regeneration. MATERIALS AND METHODS: Peel resistance and cell cytotoxicity test were performed. Four defect types in nine rabbit calvaria were randomly allocated: i) control, ii) membrane, iii) deproteinized porcine bone mineral (DPBM) covered by membrane with DOPA, and iv) DPBM covered by membrane with cyanoacrylate. Animals were sacrificed at 2 (n=4) and 8 weeks (n=5) for microcomputed tomography and histomorphometric analysis. DOPA showed low peel resistance but high cell viability. RESULT: Cyanoacrylate and DOPA groups showed significantly higher mineralized tissue volume (MTV) compared to control and membrane groups at 2 weeks (P < 0.05). At 8 weeks, DOPA group showed the highest MTV. Significantly higher new bone area was found in DOPA group at 8 weeks (P < 0.05). Bone formation increased from 2 to 8 weeks in DOPA group (P < 0.05). CONCLUSION: DOPA showed high cell viability and in vivo study revealed predictable performance in bone regeneration.
