Effect of apoptosis on G361 cells by Cimicifuga rhizoma extract
10.11149/jkaoh.2019.43.2.72
- Author:
Byul Bo Ra CHOI
1
;
Gyoo Cheon KIM
;
Jin Woo HONG
;
Sang Rye PARK
Author Information
1. Feagle Co., Ltd., Yangsan, Korea.
- Publication Type:Original Article
- Keywords:
Apoptosis;
Cimicifuga rhizoma;
Melanoma
- MeSH:
Apoptosis Inducing Factor;
Apoptosis;
Blotting, Western;
Caspase 3;
Caspase 9;
Cell Death;
Cell Line;
Cimicifuga;
Cytochromes c;
Humans;
Immunohistochemistry;
Keratinocytes;
Melanoma;
Microscopy, Confocal
- From:Journal of Korean Academy of Oral Health
2019;43(2):72-77
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361). METHODS: Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy. RESULTS: The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 µg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected. CONCLUSIONS: This study suggests that Cimicifuga rhizoma extract causes apoptosis of human melanoma cells through the intrinsic apoptotic pathway.
