Expression of Class I and Class II a/b Histone Deacetylase is Dysregulated in Hypertensive Animal Models.
- Author:
Hae Jin KEE
1
;
Gwi Ran KIM
;
Ming Quan LIN
;
Sin Young CHOI
;
Yuhee RYU
;
Li JIN
;
Zhe Hao PIAO
;
Myung Ho JEONG
Author Information
- Publication Type:Original Article
- Keywords: Hypertension; Spontaneously hypertensive rats; L-NAME; Angiotensin II; Class II histone deacetylases
- MeSH: Angiotensin II; Animals*; Blood Pressure; Histone Deacetylases*; Histones*; Humans; Hypertension; Hypertension, Pulmonary; Mice; Models, Animal*; NG-Nitroarginine Methyl Ester; Rats; Rats, Inbred SHR
- From:Korean Circulation Journal 2017;47(3):392-400
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND AND OBJECTIVES: Dysregulation of histone deacetylase expression and enzymatic activity is associated with a number of diseases. It has been reported that protein levels of histone deacetylase (HDAC)1 and HDAC5 increase during human pulmonary hypertension, and that the enzymatic activity of HDAC6 is induced in a chronic hypertensive animal model. This study investigated the protein expression profiles of class I and II a/b HDACs in three systemic hypertension models. SUBJECTS AND METHODS: We used three different hypertensive animal models: (i) Wistar-Kyoto rats (n=8) and spontaneously hypertensive rats (SHR; n=8), (ii) mice infused with saline or angiotensin II to induce hypertension, via osmotic mini-pump for 2 weeks, and (iii) mice that were allowed to drink L-N(G)-nitro-L-arginine methyl ester (L-NAME) to induce hypertension. RESULTS: SHR showed high systolic, diastolic, and mean blood pressures. Similar increases in systolic blood pressure were observed in angiotensin II or L-NAME-induced hypertensive mice. In SHR, class IIa HDAC (HDAC4, 5, and 7) and class IIb HDAC (HDAC6 and 10) protein expression were significantly increased. In addition, a HDAC3 protein expression was induced in SHR. However, in L-NAME mice, class IIa HDAC protein levels (HDAC4, 5, 7, and 9) were significantly reduced. HDAC8 protein levels were significantly reduced both in angiotensin II mice and in SHR. CONCLUSION: These results indicate that dysregulation of class I and class II HDAC protein is closely associated with chronic hypertension.
