Significance of CD133 as a cancer stem cell markers focusing on the tumorigenicity of pancreatic cancer cell lines.
10.4174/jkss.2011.81.4.263
- Author:
Hyun Joo LEE
1
;
Dong Do YOU
;
Dong Wook CHOI
;
Young Sil CHOI
;
Seong Joo KIM
;
Yong Sung WON
;
Hyoun Jong MOON
Author Information
1. Department of Health Science and Technology, Samsung Advanced Institute of Health Science and Technology, Sungkyunkwan University Graduate School, Seoul, Korea. dw7722.choi@samsung.com
- Publication Type:Original Article
- Keywords:
Pancreatic cancer;
Cancer stem cell;
Cell surface marker;
CD133;
Tumorigenicity
- MeSH:
Animals;
Cell Line;
Flow Cytometry;
Humans;
Lung;
Mice;
Neoplasm Metastasis;
Neoplastic Stem Cells;
Pancreatic Neoplasms;
Stem Cells;
Transplantation, Heterologous
- From:Journal of the Korean Surgical Society
2011;81(4):263-270
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The cancer stem cell hypothesis states that the capacity of a cancer to grow and propagate is dependent on a small subset of cells. To determine the significances of the cancer stem cell markers CD133, CD44, and CD24 using a comparative analysis with a focus on tumorigenicity. METHODS: Four pancreatic cancer cell lines, Capan-1, Mia-PACA-2, Panc-1, and SNU-410 were analyzed for the expressions of CD133, CD44, and CD24 by flow cytometry. The tumorigenicity was compared using tumor volumes and numbers of tumors formed/numbers of injection in nonobese diabetic severe combined deficiency mice. Fluorescence-activated cell sorting (FACS) analysis was used to confirm that xenograft explants originated from human pancreatic cancer cells. RESULTS: CD133 was positive in only Capan-1, CD44 positive in all, CD24 partially positive in Panc-1. After injecting 2 x 10(6) cells, all mice administered Capan-1 or Mia-Paca-2 developed tumors, 3 of 5 administered Panc-1 developed tumors, but no mouse administered SNU-410 developed any tumors. The volumes of Capan-1 tumors were seven times larger than those of Mia-Paca-2 tumors. When 2 x 10(5) or 2 x 10(4) of Capan-1 or Mia-Paca-2 was injected, tumors developed in all Capan-1 treated mice, but not in Mia-Paca-2 treated mice. Furthermore, xenograft explants of Capan-1 expressed CD133+CD44+ and Capan-1 injected mice developed lung metastasis. FACS analysis showed that xenograft explants originated from human pancreatic cancer cell lines. CONCLUSION: CD133 positive cells have higher tumorigenic and metastatic potential than CD44 and CD24 positive cells, which suggests that CD133 might be a meaningful cell surface marker of pancreatic cancer stem cells.
