Esculetin Prevents the Induction of Matrix Metalloproteinase-1 by Hydrogen Peroxide in Skin Keratinocytes
10.15430/JCP.2019.24.2.123
- Author:
Ao Xuan ZHEN
1
;
Mei Jing PIAO
;
Kyoung Ah KANG
;
Pincha Devage Sameera Madushan FERNANDO
;
Hee Kyoung KANG
;
Young Sang KOH
;
Jin Won HYUN
Author Information
1. Department of Biochemistry, Jeju National University School of Medicine, Jeju, Korea. jinwonh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Esculetin;
Reactive oxygen species;
Matrix metalloproteinase-1;
Aging
- MeSH:
Aging;
Blotting, Western;
Calcium;
Fluorescence;
Humans;
Hydrogen Peroxide;
Hydrogen;
Keratinocytes;
Matrix Metalloproteinase 1;
Oxidative Stress;
Reactive Oxygen Species;
RNA, Messenger;
Skin;
Transcription Factor AP-1
- From:Journal of Cancer Prevention
2019;24(2):123-128
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes. METHODS: Human keratinocytes were pretreated with esculetin for 30 minutes and treated with H₂O₂. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay. RESULTS: Esculetin prevented H₂O₂-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity. CONCLUSIONS: Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins.
