Induction of Apoptosis Scutellaria baicalensis Georgi Root Extract by Inactivation of the Phosphatidyl Inositol 3-kinase/Akt Signaling Pathway in Human Leukemia U937 Cells
10.15430/JCP.2019.24.1.11
- Author:
Eun Ok CHOI
1
;
Hye Jin HWANG
;
Yung Hyun CHOI
Author Information
1. Department of Biochemistry, Dong-Eui University College of Korean Medicine, Busan, Korea. choiyh@deu.ac.kr
- Publication Type:Original Article
- Keywords:
Scutellaria baicalensis;
U937 cells;
Apoptosis;
Caspase;
PI3K/Akt
- MeSH:
Apoptosis;
Blotting, Western;
Caspase 8;
Caspase 9;
Cell Survival;
Down-Regulation;
Ethanol;
Flow Cytometry;
Humans;
Leukemia;
Ligands;
Medicine, Traditional;
Membrane Potential, Mitochondrial;
Phosphatidylinositols;
Receptors, Death Domain;
Scutellaria baicalensis;
Scutellaria;
U937 Cells;
Up-Regulation
- From:Journal of Cancer Prevention
2019;24(1):11-19
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The roots of Scutellaria baicalensis Georgi (Labiatae) have been widely used in traditional medicine for treatment of various diseases. In this study, we investigated the effects of ethanol extracts of S. baicalensis roots (EESB) on the growth ofn human leukemia U937 cells. METHODS: The effect of EESB on cell viability was measured by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Apoptosis was determined using 4,6-diamidino-2-phenyllindile staining and flow cytometry. The effects of EESB on the expression of regulatory proteins of apoptosis and phosphatidyl inositol 3-kinase (PI3K)/Akt signaling were determined by Western blotting. Caspase activity and mitochondrial membrane potential (MMP) were measured using flow cytometric analysis.
