Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties
10.4167/jbv.2019.49.3.124
- Author:
Dong Kun YANG
1
;
Ha Hyun KIM
;
Eun Jin LEE
;
Jae Young YOO
;
Soon Seek YOON
;
Jungwon PARK
;
Chae Hyun KIM
;
Ho Ryoung KIM
Author Information
1. Viral Disease Research Division, Animal and Plant Quarantine Agency, MAFRA, Gimcheon, 39660, Republic of Korea. yangdk@korea.kr
- Publication Type:Original Article
- Keywords:
Canine adenovirus type 1;
Genetic characterization;
Vaccine
- MeSH:
Adenoviridae;
Adenoviruses, Canine;
Animals;
Base Sequence;
Canada;
Dogs;
Erythrocytes;
Fluorescence;
Fluorescent Antibody Technique;
Guinea Pigs;
Hemagglutination;
Hepatitis;
Madin Darby Canine Kidney Cells;
Microscopy, Electron;
Quality Control
- From:Journal of Bacteriology and Virology
2019;49(3):124-132
- CountryRepublic of Korea
- Language:English
-
Abstract:
Canine adenovirus type 1 (CAV-1) infection results in hepatitis in dogs. In this study, we investigated the biologic and genetic characteristics of the CAV-1 vaccine strain (CAV1V) to improve quality control about CAV vaccine. The identity of CAV1V as CAV-1 was confirmed based on its cytopathic effects and the results of hemagglutination (HA) and immunofluorescence assays, and electron microscopy. The CAV1V strain reached 10(7.5) TCID(50)/mL in MDCK cells at 4 days post-inoculation and exhibited hemmagglutination activity of 256 U using guinea pig erythrocytes. Intranuclear fluorescence in the infected cells was observed and typical adenoviruses were observed in electon microscope. CAV1V strain was identified as a CAV-1 strain by nucleotide sequence analysis. In a comparison of the nucleotide sequences of the fiber genes of several CAV strains, CAV1V showed the highest similarity (99.8%) with the GLAXO strain, which was isolated in Canada. Our biological characterization of CAV1V will facilitate quality control of the canine hepatitis vaccine.
