Histopathological Evaluation of the Efficacy for Plant-produced E2 Protein Vaccine against Classical Swine Fever Virus (CSFV) in Piglets
10.4167/jbv.2019.49.3.133
- Author:
Sun Young KIM
1
;
Bo Kyoung JUNG
;
Gyu Nam PARK
;
Hye Ran KIM
;
Dong Jun AN
;
Eun Ju SHON
;
Kyung Soo CHANG
Author Information
1. Department of Pathology, Dongnam Institute of Radiological and Medical Sciences, Busan 46033, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Nicotiana benthamiana;
Plant-derived vaccine;
Classical swine fever virus;
Histopathological change
- MeSH:
Animals;
Antibodies;
Classical swine fever virus;
Classical Swine Fever;
Depression;
Swine;
Tobacco;
Vaccination;
Vaccines, Synthetic
- From:Journal of Bacteriology and Virology
2019;49(3):133-140
- CountryRepublic of Korea
- Language:English
-
Abstract:
Classical swine fever (CSF), previously known as hog cholera, remains one of the most important swine-related contagious diseases worldwide. In order to eradicate classical swine fever virus (CSFV), it is commonly used in LOM-850 strain as a live attenuated CSF vaccine. However, there are symptoms of vaccination, such as the depression of feed intake, and difficulty of differentiation between infected and vaccinated hosts is impossible based on the antibodies induced. Nicotiana benthamiana were considered as an alternative to the production of recombinant vaccines on account of higher yields and levels of soluble protein than other models and crops in protein recombinant products. This study was conducted to evaluate histopathological validation of the plant-produced E2 fusion protein (ppE2) in piglets. The piglets were challenged by an injection of YC11WB strain in 7 days, 11 days and 14 days after one shot of the vaccination. The histopathological examination indicated that ppE2 can protect against lethal CSFV challenge at least 11 days of vaccination in piglets. These data suggest that the ppE2 can be an effective vaccine against CSFV in piglets.
