Preparation and characterization of rutile phase TiO₂ nanoparticles and their cytocompatibility with oral cancer cells
10.11620/IJOB.2019.44.3.108
- Author:
Vu Phuong DONG
1
;
Nguyen Thi Kieu TRANG
;
Hoon YOO
Author Information
1. Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University, Gwangju 61452, Republic of Korea. hoon_yoo@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Titanium dioxide nanoparticles;
Rutile;
Cytocompatibility;
FaDu cells;
YD-15 cells
- MeSH:
Dimethyl Sulfoxide;
Dynamic Light Scattering;
Hydrodynamics;
Hydrolysis;
Microscopy, Electron, Scanning;
Microscopy, Electron, Transmission;
Mouth Neoplasms;
Nanoparticles;
Particle Size;
Pigmentation;
Spectrometry, X-Ray Emission;
Spectrum Analysis;
Titanium;
Tooth;
X-Ray Diffraction
- From:International Journal of Oral Biology
2019;44(3):108-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, rutile phase titanium dioxide nanoparticles (R-TiO₂ NPs) were prepared by hydrolysis of titanium tetrachloride in an aqueous solution followed by calcination at 900℃. The composition of R-TiO₂ NPs was determined by the analysis of X-ray diffraction data, and the characteristic features of R-TiO₂ NPs such as the surface functional group, particle size, shape, surface topography, and morphological behavior were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy, transmission electron microscopy, dynamic light scattering, and zeta potential measurements. The average size of the prepared R-TiO₂ NPs was 76 nm, the surface area was 19 m²/g, zeta potential was −20.8 mV, and average hydrodynamic diameter in dimethyl sulfoxide (DMSO)–H₂O solution was 550 nm. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and morphological observations revealed that R-TiO₂ NPs were cytocompatible with oral cancer cells, with no inhibition of cell growth and proliferation. This suggests the efficacy of R-TiO₂ NPs for the aesthetic white pigmentation of teeth.
