Effect of Garcinia mangostana L. and propolis extracts on the inhibition of inflammation and alveolar bone loss in ligature-induced periodontitis in rats
10.11620/IJOB.2019.44.2.55
- Author:
Se Jin SUNG
1
;
Kyung Min KANG
;
Kyung Hyun LEE
;
So Young YOO
;
Joong Ki KOOK
;
Dae Sung LEE
;
Sang Joun YU
Author Information
1. Department of Periodontology, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea. sjyu78@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Mangosteen extract complex;
Anti-inflammatory;
Alveolar bone loss
- MeSH:
Alveolar Bone Loss;
Animals;
Enzyme-Linked Immunosorbent Assay;
Garcinia mangostana;
Garcinia;
Gingiva;
Inflammation;
Interleukin-8;
Matrix Metalloproteinase 8;
Molar;
Nitric Oxide Synthase Type II;
Periodontitis;
Porphyromonas gingivalis;
Propolis;
Prostaglandin-Endoperoxide Synthases;
Rats;
Silk;
Tooth
- From:International Journal of Oral Biology
2019;44(2):55-61
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to evaluate the effect of mangosteen extract complex (MEC; Garcinia mangostana L. and propolis extracts) on the inhibition of inflammation and prevention of alveolar bone loss using a ligature-induced periodontitis model. Rat molars were ligatured with silk, and 1 µg/mL of lipopolysaccharide of Porphyromonas gingivalis was injected into the buccal and palatal gingivae of the teeth with or without treatment with the MEC. Changes in the expression levels of prostaglandin E₂ (PGE₂), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), matrix metalloproteinase-8 (MMP-8), cyclooxygenase (COX)-1, and COX-2 in gingival tissues were evaluated using enzyme-linked immunosorbent assays. Alveolar bone loss around the ligated molars was examined using micro-computed tomography. The expression levels of PGE₂, IL-8, iNOS, MMP-8, COX-1, and COX-2 in gingival tissues were significantly reduced in the group treated with a mixture of 16 µg of mangosteen extract powder and 544 µg of propolis extract powder (ligation [Lig] + lipopolysaccharide extracted from P. gingivalis KCOM 2804 [L] + MEC 1:34). Additionally, alveolar bone loss was significantly reduced in the Lig + L + MEC 1:34 group compared with that in other groups. These results indicate that the MEC could be useful in preventing and treating periodontitis.
