Intravenous Immunoglobulin Controls Th17 Cell-Mediated Osteoclastogenesis
- Author:
Kyoung Woon KIM
1
;
Hae Rim KIM
;
Bo Mi KIM
;
Ji Yeon WON
;
Kyung Ann LEE
;
Sang Heon LEE
Author Information
- Publication Type:Original Article
- Keywords: IVIg; Osteoclastogenesis; RANK ligand; IL-17; Th17 cells
- MeSH: Cytokines; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Immunoglobulins; Immunoglobulins, Intravenous; Interleukin-17; Macrophage Colony-Stimulating Factor; Monocytes; Osteoclasts; RANK Ligand; Real-Time Polymerase Chain Reaction; T-Lymphocytes; T-Lymphocytes, Regulatory; Th1 Cells; Th17 Cells
- From:Immune Network 2019;19(4):e27-
- CountryRepublic of Korea
- Language:English
- Abstract: The purpose of this study was to determine the regulatory role of intravenous Ig (IVIg) in Th17 cytokine–induced RANK ligand (RANKL) expression and osteoclast (OC) differentiation from OC precursors (pre-OC). Human CD14⁺ monocytes were isolated and stimulated by Th17 cytokines (IL-17, IL-21, and IL-22) and RANKL expression was investigated using a real-time PCR. CD14⁺ monocytes were incubated with RANKL, Th17 cytokines, and M-CSF, with/without IVIg, and OC differentiation was determined by counting tartrate-resistant acid phosphatase-positive multinucleated cells. OC differentiation was investigated after monocytes were cocultured with Th17 cells in the presence of IVIg. Th17 cell differentiation was determined using enzyme-linked immunosorbent assay and flow cytometry after CD4⁺ T cells were cultured with IVIg under Th17 condition. Th17 cytokines stimulated monocytes to express RANKL and IVIg suppressed the Th17 cytokine-induced RANKL expression. OCs were differentiated when pre-OC were cocultured with RANKL or Th17 cytokines and IVIg reduced the osteoclastogenesis. IVIg also decreased osteoclastogenesis when pre-OC were cocultured with Th17 cells. IVIg decreased both Th17 and Th1 cell differentiation while it did not affect Treg cell differentiation. In summary, IVIg inhibited Th17 cytokine-induced RANKL expression and OC differentiation. IVIg reduced osteoclastogenesis when monocytes were cocultured with Th17 cells. IVIg also reduced Th17 polarization. IVIg could be a new therapeutic option for Th17 cell–mediated osteoclastogenesis.
