Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer
- Author:
Soo Young CHO
1
Author Information
- Publication Type:Original Article
- Keywords: breast neoplasms; ERBB signaling pathway; intratumor heterogeneity; molecular subtyping; single-cell RNA-seq
- MeSH: Breast Neoplasms; Population Characteristics; Precision Medicine; Sequence Analysis, RNA; Transcriptome; Triple Negative Breast Neoplasms
- From:Genomics & Informatics 2019;17(1):e3-
- CountryRepublic of Korea
- Language:English
- Abstract: Intratumor heterogeneity within a single tumor mass is one of the hallmarks of malignancy and has been reported in various tumor types. The molecular characterization of intratumor heterogeneity in breast cancer is a significant challenge for effective treatment. Using single-cell RNA sequencing (RNA-seq) data from a public resource, an ERBB pathway activated triple-negative cell population was identified. The differential expression of three subtyping marker genes (ERBB2, ESR1, and PGR) was not changed in the bulk RNA-seq data, but the single-cell transcriptomes showed intratumor heterogeneity. This result shows that ERBB signaling is activated using an indirect route and that the molecular subtype is changed on a single-cell level. Our data propose a different view on breast cancer subtypes, clarifying much confusion in this field and contributing to precision medicine.
