Basal Forebrain Cholinergic-induced Activation of Cholecystokinin Inhibitory Neurons in the Basolateral Amygdala
- Author:
Seungho LEE
1
;
Joung Hun KIM
Author Information
- Publication Type:Brief Communication
- Keywords: Cholecystokinin; Acetylcholine; Di-synaptic inhibition; Basolateral amygdala; Iontophoresis; Retrograde tracing
- MeSH: Acetylcholine; Action Potentials; Basal Forebrain; Basolateral Nuclear Complex; Cholecystokinin; Cholinergic Neurons; Conditioning, Operant; Iontophoresis; Memory; Neurons; Pyramidal Cells; Receptors, Nicotinic; Synapses
- From:Experimental Neurobiology 2019;28(3):320-328
- CountryRepublic of Korea
- Language:English
- Abstract: The basolateral amygdala (BLA) receives dense projections from cholinergic neurons of the basal forebrain. Acetylcholine can contributes to amygdala-dependent behaviors: formation and extinction of fear memory and appetitive instrumental learning. However, the cholinergic mechanism at the circuit level has not been defined yet. We demonstrated that cholinergic-induced di-synaptic inhibition of BLA pyramidal neurons exhibits a retrograde form of short-term synaptic inhibition, depolarization-induced suppression of inhibition (DSI). Activation of nicotinic receptors was sufficient to evoke action potentials in cholecystokinin (CCK)-positive inhibitory neurons, which strongly inhibit pyramidal neurons through their perisomatic synapses. Our cell type-specific monosynaptic retrograde tracing also revealed that CCK neurons are innervated by basal forebrain cholinergic neurons. Therefore, our data indicated that CCK inhibitory neurons mediate the cholinergic-induced di-synaptic inhibition of BLA pyramidal neurons.
