The Evaluation of Exogenous Melatonin Administration in Supraspinatus Overuse Tendinopathy in an Experimental Rat Model
10.5397/cise.2019.22.2.79
- Author:
Onur KOCADAL
1
;
Murad PEPE
;
Nalan AKYUREK
;
Zafer GUNES
;
Hatice SURER
;
Ertugrul AKSAHIN
;
Betul OGUT
;
Cem Nuri AKTEKIN
Author Information
1. Department of Orthopedics and Traumatology, Yeditepe University Faculty of Medicine, Istanbul, Turkey. onurkocadal@gmail.com
- Publication Type:Original Article
- Keywords:
Shoulder;
Rotator cuff;
Tendinopathy;
Oxidative stress;
Melatonin
- MeSH:
Adrenal Cortex Hormones;
Animals;
Biopsy;
Inflammation;
Melatonin;
Models, Animal;
Nitric Oxide Synthase Type II;
Oxidative Stress;
Plasma;
Rats;
Rotator Cuff;
Shoulder;
Tendinopathy;
Triamcinolone;
Vascular Endothelial Growth Factor A
- From:Journal of the Korean Shoulder and Elbow Society
2019;22(2):79-86
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Increased oxidative stress and inflammation play a critical role in the etiopathogenesis of chronic tendinopathy. Melatonin is an endogenous molecule that exhibits antioxidant and anti-inflammatory activity. The aim of this study was to evaluate the biochemical and histopathological effects of exogenous melatonin administrations in supraspinatus overuse tendinopathy. METHODS: Fifty rats were divided into the following four groups: cage activity, melatonin treatment, corticosteriod therapy, and control. Melatonin (10 mg/kg, intraperitoneal; twice a day) and triamcinolone (0.3 mg/kg, subacromial; weekly) were administered to the treatment groups after the overuse period. Biochemical and histopathological evaluations were performed on serum samples and biopsies obtained from rats. Plasma inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) levels were evaluated biochemically. RESULTS: The TAS, TOS, OSI, iNOS, and VEGF values were significantly lower than the pre-treatment levels in rats receiving exogenous melatonin treatment (3 or 6 weeks) (p<0.05). TOS, iNOS, VEGF, and OSI values after 3 weeks of triamcinolone administration, and TOS, VEGF, and OSI levels after 6 weeks of triamcinolone application, were significantly lower than the pre-treatment levels (p<0.05). CONCLUSIONS: Exogenous melatonin application in overuse tendinopathy reduces oxidative stress and inflammation. Melatonin might be an alternative potential molecule to corticosteroids in the treatment of chronic tendinopathy.
