Platelet-Rich Plasma Pretreatment on Grit-Blasted Titanium Alloy for Enhanced Osteogenic Differentiation of Human Adipose-Derived Stem Cells
10.4055/cios.2019.11.3.361
- Author:
Seong Hwa HONG
1
;
Jinwoo NAM
;
Hee Joong KIM
;
Jeong Joon YOO
Author Information
1. Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, Korea. jyos@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Platelet-rich plasma;
Titanium alloy;
Stem cell
- MeSH:
Alkaline Phosphatase;
Alloys;
Calcium;
Cell Count;
Collagen Type I;
Gene Expression;
Humans;
Osteocalcin;
Osteogenesis;
Platelet-Rich Plasma;
Polymerase Chain Reaction;
Reverse Transcription;
RNA, Messenger;
Stem Cells;
Subcutaneous Fat;
Thigh;
Titanium
- From:Clinics in Orthopedic Surgery
2019;11(3):361-368
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Adequate bone formation around titanium alloy implants is integral to successful implantation surgery. Stem cell-coated implants may accelerate peri-implant bone formation. This study investigates the effect of platelet-rich plasma (PRP) pretreatment on a titanium-alloy surface in terms of proliferation and osteogenic differentiation of human adipose-derived stem cells (hADSCs). METHODS: Allogenic leukocyte-depleted PRP was obtained from blood supernatants. The hADSCs were isolated from thigh subcutaneous fat tissue. Grit-blasted titanium plugs were used in two different groups. In one group, 200 µL of PRP was added to the grit-blasted titanium plugs. The hADSCs were seeded in two groups: grit-blasted titanium plugs with or without PRP. The number of hADSCs was measured after 4 hours, 3 days, and 7 days of culture using Cell Counting Kit-8. Osteogenesis of hADSCs was measured by using an alkaline phosphatase activity assay on days 7 and 14, and a calcium assay on days 14 and 21. Osteogenic gene expression was measured by using reverse transcription polymerase chain reaction analysis of alkaline phosphatase, osteocalcin, and type I collagen mRNA. The microscopic morphology of grit-blasted titanium plugs with or without PRP was examined with a field-emission scanning electron microscope using a JSM-7401F apparatus on days 1 and 7. RESULTS: Proliferation and osteogenic differentiation of hADSCs were found to be significantly higher on the grit-blasted titanium alloy preprocessed with PRP than the same alloy without pretreatment. Furthermore, a structural fibrillar mesh developed compactly on the grit-blasted titanium alloy with the PRP pretreatment. CONCLUSIONS: Our results demonstrate that a hADSC-based approach can be used for tissue-engineered peri-implant bone formation and that PRP pretreatment on the grit-blasted titanium alloy can improve proliferation and osteogenic differentiation of hADSCs.
