Prognostic utility of ADAMTS13 activity for the atypical hemolytic uremic syndrome (aHUS) and comparison of complement serology between aHUS and thrombotic thrombocytopenic purpura
- Author:
Jisu OH
1
;
Doyeun OH
;
Seon Ju LEE
;
Jeong Oh KIM
;
Nam Keun KIM
;
So Young CHONG
;
Ji Young HUH
;
Ross I BAKER
;
Author Information
- Publication Type:Original Article
- Keywords: Thrombotic thrombocytopenic purpura; Atypical hemolytic uremic syndrome; Complement; High ADAMTS13 activity; Treatment outcomes
- MeSH: Atypical Hemolytic Uremic Syndrome; Biomarkers; Complement Activation; Complement System Proteins; Enzyme-Linked Immunosorbent Assay; Humans; Mortality; Plasma; Purpura, Thrombotic Thrombocytopenic; Thrombotic Microangiopathies
- From:Blood Research 2019;54(3):218-228
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Atypical hemolytic uremic syndrome (aHUS) involves dysregulation of the complement system, but whether this also occurs in thrombotic thrombocytopenic purpura (TTP) remains unclear. Although these conditions are difficult to differentiate clinically, TTP can be distinguished by low (<10%) ADAMTS13 activity. The aim was to identify the differences in complement activation products between TTP and aHUS and investigate ADAMTS13 activity as a prognostic factor in aHUS. METHODS: We analyzed patients with thrombotic microangiopathy diagnosed as TTP (N=48) or aHUS (N=50), selected from a Korean registry (N=551). Complement activation products in the plasma samples collected from the patients prior to treatment and in 40 healthy controls were measured by ELISA. RESULTS: The levels of generalized (C3a), alternate (factor Bb), and terminal (C5a and C5b-9) markers were significantly higher (all P<0.01) in the patients than in the healthy controls. Only the factor Bb levels significantly differed (P=0.008) between the two disease groups. In aHUS patients, high normal ADAMTS13 activity (≥77%) was associated with improved treatment response (OR, 6.769; 95% CI, 1.605–28.542; P=0.005), remission (OR, 6.000; 95% CI, 1.693–21.262; P=0.004), exacerbation (OR, 0.242; 95% CI, 0.064–0.916; P=0.031), and disease-associated mortality rates (OR, 0.155; 95% CI, 0.029–0.813; P=0.017). CONCLUSION: These data suggest that complement biomarkers, except factor Bb, are similarly activated in TTP and aHUS patients, and ADAMTS13 activity can predict the treatment response and outcome in aHUS patients.
