Enrichment of Short-Chain Ceramides and Free Fatty Acids in the Skin Epidermis, Liver, and Kidneys of db/db Mice, a Type 2 Diabetes Mellitus Model
10.4062/biomolther.2018.214
- Author:
Minjeong KIM
1
;
Haengdueng JEONG
;
Buhyun LEE
;
Yejin CHO
;
Won Kee YOON
;
Ahreum CHO
;
Guideock KWON
;
Ki Taek NAM
;
Hunjoo HA
;
Kyung Min LIM
Author Information
1. Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea. kmlim@ewha.ac.kr, hha@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Lipids;
Ceramide;
Free fatty acid;
Diabetes mellitus;
Skin barrier function
- MeSH:
Animals;
Ceramides;
Diabetes Mellitus;
Diabetes Mellitus, Type 2;
Epidermis;
Fatty Acids, Nonesterified;
Humans;
Hyperglycemia;
Kidney;
Liver;
Mice;
Receptors, Cytoplasmic and Nuclear;
Skin;
Stearoyl-CoA Desaturase;
Subcutaneous Fat
- From:Biomolecules & Therapeutics
2019;27(5):457-465
- CountryRepublic of Korea
- Language:English
-
Abstract:
Patients with diabetes mellitus (DM) often suffer from diverse skin disorders, which might be attributable to skin barrier dysfunction. To explore the role of lipid alterations in the epidermis in DM skin disorders, we quantitated 49 lipids (34 ceramides, 14 free fatty acids (FFAs), and cholesterol) in the skin epidermis, liver, and kidneys of db/db mice, a Type 2 DM model, using UPLC-MS/MS. The expression of genes involved in lipid synthesis was also evaluated. With the full establishment of hyperglycemia at the age of 20 weeks, remarkable lipid enrichment was noted in the skin of the db/db mice, especially at the epidermis and subcutaneous fat bed. Prominent increases in the ceramides and FFAs (>3 fold) with short or medium chains (
