7,8,4′-Trihydroxyisoflavone, a Metabolized Product of Daidzein, Attenuates 6-Hydroxydopamine-Induced Neurotoxicity in SH-SY5Y Cells
10.4062/biomolther.2018.211
- Author:
Yong Hyun KO
1
;
Seon Kyung KIM
;
Seung Hwan KWON
;
Jee Yeon SEO
;
Bo Ram LEE
;
Young Jung KIM
;
Kwang Hyun HUR
;
Sun Yeou KIM
;
Seok Yong LEE
;
Choon Gon JANG
Author Information
1. Department of Pharmacology, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea. jang@skku.edu
- Publication Type:Original Article
- Keywords:
7,8,4′-Trihydroxyisoflavone;
6-Hydroxydopamine;
Neurotoxicity;
Apoptosis;
Parkinson's disease
- MeSH:
Apoptosis;
Caspase 3;
Caspase 9;
Catalase;
Cell Death;
Glutathione;
Glycogen Synthase;
In Vitro Techniques;
JNK Mitogen-Activated Protein Kinases;
L-Lactate Dehydrogenase;
Malondialdehyde;
Neurons;
Oxidopamine;
Parkinson Disease;
Phosphatidylinositol 3-Kinases;
Phosphotransferases;
Protein Kinases;
Soybeans;
Superoxide Dismutase;
Tyrosine 3-Monooxygenase
- From:Biomolecules & Therapeutics
2019;27(4):363-372
- CountryRepublic of Korea
- Language:English
-
Abstract:
Daidzein isolated from soybean (Glycine max) has been widely studied for its antioxidant and anti-inflammatory activities. However, the protective effects of 7,8,4′-trihydroxyisoflavone (THIF), a major metabolite of daidzein, on 6-hydroxydopamine (OHDA)-induced neurotoxicity are not well understood. In the current study, 7,8,4′-THIF significantly inhibited neuronal cell death and lactate dehydrogenase (LDH) release induced by 6-OHDA in SH-SY5Y cells, which were used as an in vitro model of Parkinson's disease (PD). Moreover, pretreatment with 7,8,4′-THIF significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) and decreased malondialdehyde (MDA) activity in 6-OHDA-induced SH-SY5Y cells. In addition, 7,8,4′-THIF significantly recovered 6-OHDA-induced cleaved caspase-3, cleaved caspase-9, cleaved poly-ADP-ribose polymerase (PARP), increased Bax, and decreased Bcl-2 levels. Additionally, 7,8,4′-THIF significantly restored the expression levels of phosphorylated c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase 1/2 (ERK 1/2), phosphatidylinositol 3-kinases (PI3K)/Akt, and glycogen synthase kinase-3 beta (GSK-3β) in 6-OHDA-induced SH-SY5Y cells. Further, 7,8,4′-THIF significantly increased the reduced tyrosine hydroxylase (TH) level induced by 6-OHDA in SH-SY5Y cells. Collectively, these results suggest that 7,8,4′-THIF protects against 6-OHDA-induced neuronal cell death in cellular PD models. Also, these effects are mediated partly by inhibiting activation of the MAPK and PI3K/Akt/GSK-3β pathways.
