4′-O-β-D-Glucosyl-5-O-Methylvisamminol Attenuates Pro-Inflammatory Responses and Protects against Oxidative Damages
10.4062/biomolther.2018.232
- Author:
Ok Kyung YOO
1
;
Young Sam KEUM
Author Information
1. Integrated Research Institute for Drug Development, College of Pharmacy, Dongguk University, Goyang 10326, Republic of Korea. keum03@dongguk.edu
- Publication Type:Original Article
- Keywords:
4′-O-β-D-glucosyl-5-O-methylvisamminol (GOMV);
Reactive oxygen species (ROS);
NF-E2-related factor 2 (NRF2);
Antioxidant response element (ARE)
- MeSH:
Antioxidant Response Elements;
Antioxidants;
Cyclooxygenase 2;
Epigenomics;
Free Radicals;
Histones;
Humans;
In Vitro Techniques;
Keratinocytes;
Macrophages;
NF-E2-Related Factor 2;
Nitric Oxide;
Nitric Oxide Synthase Type II;
Phosphorylation;
RAW 264.7 Cells
- From:Biomolecules & Therapeutics
2019;27(4):381-385
- CountryRepublic of Korea
- Language:English
-
Abstract:
We attempted to examine anti-inflammatory and anti-oxidant effects of 4′-O-β-D-glucosyl-5-O-methylvisamminol (GOMV), the first epigenetic inhibitor of histone phosphorylation at Ser10. While GOMV did not affect the viability of murine macrophage RAW 264.7 cells, it significantly suppressed lipopolysaccharide (LPS)-induced generation of prostaglandin E₂ (PGE₂) and nitric oxide (NO) through transcriptional inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). GOMV also scavenged free radicals in vitro, increased NF-E2-related factor 2 (NRF2), and activated antioxidant response element (ARE), thereby resulting in the induction of phase II cytoprotective enzymes in human keratinocyte HaCaT cells. Finally, GOMV significantly protected HaCaT cells against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative intracellular damages. Together, our results illustrate that GOMV possesses anti-inflammatory and anti-oxidant activity.
