Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
10.4062/biomolther.2018.151
- Author:
Ao Xuan ZHEN
1
;
Mei Jing PIAO
;
Yu Jae HYUN
;
Kyoung Ah KANG
;
Yea Seong RYU
;
Suk Ju CHO
;
Hee Kyoung KANG
;
Young Sang KOH
;
Mee Jung AHN
;
Tae Hoon KIM
;
Jin Won HYUN
Author Information
1. Jeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, Republic of Korea. jinwonh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Purpurogallin;
Ultraviolet B radiation;
Particulate matter 2.5;
Oxidative stress;
Human HaCaT keratinocytes
- MeSH:
Antioxidants;
Apoptosis Regulatory Proteins;
Apoptosis;
DNA;
Humans;
Keratinocytes;
Oxidative Stress;
Particulate Matter;
Phenol;
Reactive Oxygen Species
- From:Biomolecules & Therapeutics
2019;27(4):395-403
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM(2.5) severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM(2.5). Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM(2.5).
