Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats
10.4174/astr.2019.97.6.271
- Author:
Gulcin ERCAN
1
;
Rumeysa İLBAR TARTAR
;
Ali SOLMAZ
;
Osman Bilgin GULCICEK
;
Onur Olgac KARAGULLE
;
Serhat MERIC
;
Huseyin CAYOREN
;
Ramazan KUSASLAN
;
Ahu KEMIK
;
Damla GOKCEOGLU KAYALI
;
Sule CETINEL
;
Atilla CELIK
Author Information
1. Department of General Surgery, University of Health Sciences Bagcilar Training and Research Hospital, Istanbul, Turkey. ghepgul@hotmail.com
- Publication Type:Original Article
- Keywords:
Ceruletide;
NF-kappa B;
Pancreas;
Pancreatitis;
Ruscogenin
- MeSH:
Amylases;
Animals;
Ceruletide;
Intercellular Adhesion Molecule-1;
Interleukin-6;
Lipase;
Malondialdehyde;
Microscopy, Electron, Transmission;
Necrosis;
NF-kappa B;
Nitric Oxide Synthase;
Pancreas;
Pancreatitis;
Rats;
Therapeutic Uses
- From:Annals of Surgical Treatment and Research
2019;97(6):271-281
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To determine the potential protective and therapeutic effects and action mechanism of ruscogenin on cerulein-induced acute pancreatitis (AP) model in rats. METHODS: Overall, 32 rats were attenuated to the sham (2-mL/kg/day isotonic solution for 4 weeks), control (20-µg/kg cerulein-induced AP for 12 hours), prophylaxis groups (cerulein-induced AP following 3-mL/kg/day ruscogenin for 4 weeks) and treatment (3-mL/kg/day ruscogenin following cerulein-induced AP for 12 hours). Blood samples were collected for biochemical analysis of nitric oxide synthase 1 (NOS1/neuronal NOS), malondialdehyde (MDA) and intercellular adhesion molecule 1 (ICAM-1). After sacrification, pancreas tissues were collected and prepared for light microscopic (hematoxylin and eosin), immunohistochemical (nuclear factor kappa B) and biochemical analysis (tumor necrosis factor-alpha [TNF-α], interleukin-6 and 1β [IL-6 and IL-1β], CRP, high-sensitivity CRP [hs-CRP] amylase, lipase, and ICAM-1). Ultrastructural analysis was performed by transmission electron microscopy. RESULTS: The protective and therapeutic actions of ruscogenin were accomplished by improvements in histopathology, by decreasing blood cytokine levels of CRP, hs-CRP levels, TNF-α, IL-6, IL-1β, ICAM-1, by reducing the pancreatic enzymes amylase and lipase in blood, and by suppressing the expression of nuclear factor kappa B, ICAM-1, and NOS-1, but not MDA in pancreatic tissues. Ruscogenin also improved cerulein-induced ultrastructural degenerations in endocrine and exocrine cells, especially in treatment group. CONCLUSION: The present findings have demonstrated the beneficial protective and therapeutical effects of ruscogenin, nominating it as a highly promising supplementary agent to be considered in the treatment of AP, and even as a protective agent against the damages induced by disease.
