Urinary transglutaminase 2 as a potent biomarker to predict interstitial fibrosis and tubular atrophy of kidney allograft during early posttransplant period in deceased donor kidney transplantation
10.4174/astr.2019.97.1.27
- Author:
Jee Yeon KIM
1
;
Yu Mee WEE
;
Monica Young CHOI
;
Hey Rim JUNG
;
Ji Yoon CHOI
;
Hyun Wook KWON
;
Joo Hee JUNG
;
Yong Mee CHO
;
Heounjeong GO
;
Minkyu HAN
;
Young Hoon KIM
;
Duck Jong HAN
;
Sung SHIN
Author Information
1. Division of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. sshin@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Biomarkers;
Transglutaminase 2;
Kidney transplantation
- MeSH:
Allografts;
Atrophy;
Biomarkers;
Biopsy;
Extracellular Matrix;
Fibrosis;
Humans;
Inflammation;
Kidney Transplantation;
Kidney;
Methods;
Prospective Studies;
Proteoglycans;
Syndecan-4;
Tissue Donors;
Transplant Recipients
- From:Annals of Surgical Treatment and Research
2019;97(1):27-35
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Transglutaminase type 2 (TG2) is an extracellular matrix crosslinking enzyme with a pivotal role in kidney fibrosis. We tested whether quantification of urinary TG2 may represent a noninvasive method to estimate the severity of kidney allograft fibrosis. METHODS: We prospectively collected urine specimens from 18 deceased donor kidney transplant recipients at 1-day, 7-day, 1-month, 3-month, and 6-month posttransplant. In addition, kidney allograft tissue specimens at 0-day and 6-month posttransplant were sampled to analyze the correlation of urinary TG2 and kidney allograft fibrosis. RESULTS: Thirteen recipients had increased interstitial fibrosis and tubular atrophy (IFTA) scores at the 6-month protocol biopsy (IFTA group). The mean level of urinary TG2 in the IFTA group was higher compared to that of 5 other recipients without IFTA (no IFTA group). Conversely, the mean level of urinary syndecan-4 in the IFTA group was lower than levels in patients without IFTA. In the IFTA group, double immunofluorescent staining revealed that TG2 intensity was significantly upregulated and colocalizations of TG2/heparin sulfate proteoglycan and nuclear syndecan-4 were prominent, usually around tubular structures. CONCLUSION: Urinary TG2 in early posttransplant periods is a potent biomarker for kidney allograft inflammation or fibrosis.
