The Value of MicroRNAs as an Indicator of the Severity and the Acute Phase of Spinal Cord Injury
10.5535/arm.2019.43.3.328
- Author:
Junyoung PARK
1
;
Dongsoo YI
;
Jiyoon JANG
;
Jiseon HONG
Author Information
1. Department of Rehabilitation Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, Korea. sadocean17@naver.com
- Publication Type:Original Article
- Keywords:
Spinal cord;
Spinal cord injuries;
MicroRNAs;
Gene expression regulation;
Therapeutics
- MeSH:
Behavior Rating Scale;
Catheters;
Gene Expression Regulation;
Humans;
Male;
MicroRNAs;
Polymerase Chain Reaction;
Rats, Wistar;
RNA;
Spinal Cord Injuries;
Spinal Cord
- From:Annals of Rehabilitation Medicine
2019;43(3):328-334
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To assess the role of miRNA-21 and miRNA-223 in a balloon-compression model of spinal cord injury (SCI). METHODS: A total of 50 male Wistar rats (n=50) were divided into the three groups: the group A (n=15, insertion of the unflated Fogarty balloon catheter), the group B (n=15, insertion of the Fogarty balloon catheter at a volume of 20 μL) and the group C (n=15, insertion of the Fogarty balloon catheter at a volume of 50 μL). After the behavioral test, RNA isolation, microRNA expression profiling using microarrays and quantitative polymerase chain reaction, measurements were compared between the three groups. RESULTS: Despite a lack of significant differences in time-dependent changes in miRNA-21 expression levels between the three groups at 4 hours, there were significant differences in them at 1, 3, and 7 days (p<0.05). Moreover, there were significant differences in time-dependent changes in miRNA-223 expression levels between the three groups at 4 hours and 1, 3, and 7 days (p<0.05). Furthermore, miRNA-223 expression levels reached the highest at 1 day but were decreased with time thereafter in all the three groups. CONCLUSION: Expression levels of miRNA-21 and miRNA-223 might be associated with the severity and acute phase of SCI, respectively. It is mandatory, however, to analyze changes in levels of inflammatory markers and the relevant biological pathways.
