Comparison of Six Commercial Diagnostic Tests for the Detection of Dengue Virus Non-Structural-1 Antigen and IgM/IgG Antibodies
10.3343/alm.2019.39.6.566
- Author:
Hyeyoung LEE
1
;
Ji Hyeong RYU
;
Hye Sun PARK
;
Ki Hyun PARK
;
Hyunjoo BAE
;
Sojeong YUN
;
Ae Ran CHOI
;
Sung Yeon CHO
;
Chulmin PARK
;
Dong Gun LEE
;
Jihyang LIM
;
Jehoon LEE
;
Seungok LEE
;
Soyoung SHIN
;
Haeil PARK
;
Eun Jee OH
Author Information
1. Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. ejoh@catholic.ac.kr
- Publication Type:Brief Communication
- Keywords:
Dengue virus;
Diagnosis;
Rapid diagnostic tests;
ELISA;
Performance
- MeSH:
Antibodies;
Chungcheongnam-do;
Dengue Virus;
Dengue;
Diagnosis;
Diagnostic Tests, Routine;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunoglobulin G;
Immunoglobulin M
- From:Annals of Laboratory Medicine
2019;39(6):566-571
- CountryRepublic of Korea
- Language:English
-
Abstract:
ELISAs and rapid diagnostic tests (RDTs) are widely used for diagnosing dengue virus (DENV) infection. Using 138 single blood samples, we compared the ability to detect non-structural (NS)-1 antigen and anti-DENV IgM/IgG antibodies among (1) DENV Detect NS1 ELISA, DENV Detect IgM capture ELISA and DENV Detect IgG ELISA (InBios International, Inc.); (2) Anti-Dengue virus IgM Human ELISA and Anti-Dengue virus IgG Human ELISA (Abcam); (3) Dengue virus NS1 ELISA, Anti-Dengue virus ELISA (IgM) and Anti-Dengue virus ELISA (IgG) (Euroimmun); (4) Asan Easy Test Dengue NS1 Ag 100 and Asan Easy Test Dengue IgG/IgM (Asan Pharm); (5) SD BIOLINE Dengue Duo (Standard Diagnostics); and (6) Ichroma Dengue NS1 and Ichroma Dengue IgG/IgM (Boditech Med). For NS1 antigen detection, InBios and Euroimmun showed higher sensitivities (100%) than the RDTs (42.9–64.3%). All tests demonstrated variable sensitivities for IgM (38.1–90.5%) and IgG (65.7–100.0%). InBios and Boditech Med demonstrated higher sensitivity (95.6% and 88.2%, respectively) than the other tests for combined NS1 antigen and IgM antibody. Five NS1 antigen tests had good agreement (92.8–98.6%) without showing positivity for chikungunya. However, all IgG tests demonstrated potential false-positivity with variable ranges. Clinical laboratories should note performance variations across tests and potential cross-reactivity.
