RE-ORGA, a Korean Herb Extract, Can Prevent Hair Loss Induced by Dihydrotestosterone in Human Dermal Papilla Cells
- Author:
Myung Gyun KANG
1
;
Daeui PARK
;
Hyoung Yun HAN
;
Hyeeun SHIM
;
Yoonjung HONG
;
Jiyeon MOON
;
Seokjoo YOON
;
Bosun KWON
Author Information
- Publication Type:Original Article
- Keywords: Androgenic alopecia; Dihydrotestosterone; Finasteride; Herbal extract; 5 alpha-reductase
- MeSH: Alopecia; Blotting, Western; Cell Count; Cell Proliferation; Cholestenone 5 alpha-Reductase; Dihydrotestosterone; Down-Regulation; Finasteride; Hair; Humans; Interleukin-6; Protein-Tyrosine Kinases; Receptors, Androgen; RNA, Messenger; Tumor Necrosis Factor-alpha; Up-Regulation
- From:Annals of Dermatology 2019;31(5):530-537
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Androgenic alopecia (AGA) is the most common type of hair loss. It is likely inherited genetically and is promoted by dihydrotestosterone. 5α-reductase has been proven a good target through finasteride use. However, the pathogenesis of AGA cannot be fully explained based only on dihydrotestosterone levels. OBJECTIVE: To identify similar hairloss inhibition activity of RE-ORGA with mode of action other than finasteride. METHODS: We prepared RE-ORGA from Korean herb mixtures. We performed MTT assays for cytotoxicity, Cell Counting Kit-8 assays for cell proliferation, and western blot to identify expression levels of 5α-reductase and Bax. RNA-sequencing was performed for the expression patterns of genes in dihydrotestosterone-activated pathways. Anti-inflammatory activity was also assessed by the expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin 6. RESULTS: REORGA could promote the proliferation of human dermal papilla cells and showed low cytotoxicity. It also inhibited the expression of 5α-reductases and Bax in the cells. RNA-sequencing results verified that the mRNA expressions of SRD5A1, Bax, transforming growth factor-beta 1 (TGF-β1), and TGF-β1 induced transcript 1 (TGFβ1I1) were decreased, whereas expression of protein tyrosine kinase 2 beta (PTK2β) was more elevated. REORGA also showed anti-inflammatory activity through decreased mRNA levels of TNF-α. CONCLUSION: Transcriptionally, up-regulation of PTK2β and concomitant down-regulation of TGFβ1I1 imply that RE-ORGA can modulate androgen receptor sensitivity, decreasing the expression of 5α-reductase type II and Bax together with TGF-β1 transcripts; RE-ORGA also showed partial anti-inflammatory activity. Overall, RE-ORGA is expected to alleviate hair loss by regulating 5α-reductase activity and the receptor's androgen sensitivity.
