Antimicrobial Susceptibility and Genetic Analysis of Campylobacter jejuni Isolated from Diarrhea Patients in Busan
- Author:
Sun Hee PARK
1
;
Byeong Jun KIM
;
Young Hee KWON
;
In Yeong HWANG
;
Gyung Hye SUNG
;
Eun Hee PARK
;
Sung Hyun JIN
Author Information
- Publication Type:Original Article
- Keywords: Antimicrobial susceptibility; Campylobacter jejuni; Pulsed-field gel electrophoresis
- MeSH: Azithromycin; Busan; Campylobacter jejuni; Campylobacter; Chloramphenicol; Ciprofloxacin; Diarrhea; Disease Outbreaks; DNA; Electrophoresis, Gel, Pulsed-Field; Erythromycin; Foodborne Diseases; Gastroenteritis; Genotype; Gentamicins; Humans; Incidence; Korea; Methods; Nalidixic Acid; Quinolones; Streptomycin; Tetracycline
- From:Annals of Clinical Microbiology 2019;22(2):42-49
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Campylobacter jejuni is an important food-borne pathogen that causes human gastroenteritis. This study was conducted to investigate the incidence of isolation, antimicrobial susceptibility pattern, and C. jejuni genotype from diarrhea patients in Busan, Korea. METHODS: A total of 97 C. jejuni were isolated from diarrhea patients during five food-borne outbreaks from 2014 to September 2017. Antimicrobial susceptibility tests were carried out by the broth microdilution method for ciprofloxacin (CIP), nalidixic acid (NAL), tetracycline (TET), chloramphenicol, azithromycin (AZI), erythromycin (ERY), streptomycin (STR), gentamicin, and telithromycin. To investigate C. jejuni genotypes, pulsed-field gel electrophoresis (PFGE) profile analysis was performed. RESULTS: The isolation rate of C. jejuni was 2.0% for the last 4 years and increased annually. Antimicrobial resistance rates of C. jejuni were shown to be in the order of NAL (90.9%), CIP (89.4%), TET (13.6%), AZI (3.0%), ERY (3.0%), and STR (1.5%). The proportion of multidrug-resistance was 18.2%, and they commonly contained quinolones (CIP-NAL). Analysis of PFGE patterns of SmaI-restricted DNA of C. jejuni isolates showed 17 clusters; cluster 11 was the major genotype pattern. CONCLUSION: This study will provide useful data for the proper use of antimicrobials and the management of resistant C. jejuni. Also it will help to provide data for the epidemiological investigation of foodborne diseases caused by C. jejuni, which is expected to increase in the future.
