Induction of Melanoma Cell-Selective Apoptosis Using Anti-HER2 Antibody-Conjugated Gold Nanoparticles
10.3349/ymj.2019.60.6.509
- Author:
Hyeon Jun JEON
1
;
Byul Bo Ra CHOI
;
Kwang Ha PARK
;
Dae Seok HWANG
;
Uk Kyu KIM
;
Gyoo Cheon KIM
Author Information
1. Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea. kuksjs@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
HER2;
melanoma cells;
cell cycle arrest;
apoptosis;
conjugation
- MeSH:
Actins;
Apoptosis Inducing Factor;
Apoptosis;
Blotting, Western;
Caspase 3;
Caspases;
Cell Adhesion;
Cell Cycle;
Cell Cycle Checkpoints;
Cell Death;
Cyclin A;
Cyclin D1;
Cyclin E;
Cyclins;
Cytochromes c;
Cytoplasm;
DNA Fragmentation;
Down-Regulation;
Flow Cytometry;
Fluorescent Antibody Technique;
Focal Adhesions;
Melanoma;
Mitochondria;
Nanoparticles;
Phosphotransferases;
Receptor, Epidermal Growth Factor;
Up-Regulation
- From:Yonsei Medical Journal
2019;60(6):509-516
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study was conducted to verify the induction and mechanism of selective apoptosis in G361 melanoma cells using anti-HER2 antibody-conjugated gold nanoparticles (GNP-HER2). MATERIALS AND METHODS: Following GNP-HER2 treatment of G361 cells, cell cycle arrest and apoptosis were measured by WST-1 assay, Hemacolor staining, Hoechst staining, immunofluorescence staining, fluorescence-activated cell sorting analysis, and Western blotting.
