Upregulation of miR-27b Facilitates Apoptosis of TNF-α-Stimulated Fibroblast-Like Synoviocytes
10.3349/ymj.2019.60.6.585
- Author:
Shangwen LEI
1
;
Guanghua CHEN
;
Liang DENG
;
Jianying HE
Author Information
1. Department of Rheumatism and Immunology, Gansu Provincial Hospital, Lanzhou, Gansu, China.
- Publication Type:Original Article
- Keywords:
miR-27b;
rheumatoid arthritis;
NF-κB signaling pathway;
IL-1β
- MeSH:
Apoptosis;
Arthritis, Rheumatoid;
Blotting, Western;
Caspase 3;
Cell Survival;
Flow Cytometry;
Luciferases;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Tumor Necrosis Factor-alpha;
Up-Regulation
- From:Yonsei Medical Journal
2019;60(6):585-591
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to explore the function of microRNA-27b (miR-27b) in fibroblast-like synoviocytes (FLSs) stimulated by tumor necrosis factor α (TNF-α). MATERIALS AND METHODS: mRNA expression of miR-27b in FLS cells (MH7A) treated with or without TNF-α was determined by q-PCR. MiR-27b mimics was transfected into MH7A cells to upregulate miR-27b expression. MTT assay and flow cytometry analysis were performed to investigate the effect of miR-27b on MH7A cell viability and apoptosis. The targets of miR-27b were predicted by TargetScan. The direct regulation of miR-27b on IL-1β expression was verified by luciferase assay. The protein expression levels of apoptosis-related proteins, IL-1β, and NF-κB signaling-related proteins were detected by Western blot. RESULTS: We discovered that miR-27b expression was decreased in MH7A cells stimulated by TNF-α. Upregulation of miR-27b by miR-27b mimics significantly inhibited the proliferation and promoted the apoptosis of TNF-α-stimulated MH7A cells. Consistently, upregulation of miR-27 decreased the level of Bcl-2 and increased Bax and caspase-3 expression in MH7A cells stimulated by TNF-α. Luciferase assay revealed that IL-1β was indeed a target of miR-27b. By quantitative real-time PCR and Western blot, we found that the expression of IL-1β is negatively regulated by miR-27b. Moreover, the NF-κB signaling pathway was significantly inhibited by miR-27b. CONCLUSION: Taken together, our results illustrated that enhanced miR-27b expression results in the suppression of proliferation and the promotion of apoptosis in FLSs stimulated by TNF-α, partially by regulating IL-1β expression and NF-κB signaling.
