Adipose-Derived Stem Cell Transplantation Inhibits Vascular Inflammatory Responses and Endothelial Dysfunction in Rats with Atherosclerosis
10.3349/ymj.2019.60.11.1036
- Author:
Mingqiang FAN
1
;
Jing BAI
;
Tao DING
;
Xiangxiang YANG
;
Qiaoke SI
;
Dengmei NIE
Author Information
1. Department of Dardiovascular, Pingliang People's Hospital, Pingliang, China.
- Publication Type:Original Article
- Keywords:
ADSC;
atherosclerosis;
endothelial dysfunction;
inflammatory responses;
NF-κB pathway
- MeSH:
Animals;
Aorta, Thoracic;
Atherosclerosis;
Blotting, Western;
C-Reactive Protein;
Cholesterol;
Endothelin-1;
Enzyme-Linked Immunosorbent Assay;
Fluorescent Antibody Technique;
Intercellular Adhesion Molecule-1;
Interleukin-6;
Lipoproteins;
Phosphorylation;
Plaque, Atherosclerotic;
Rats;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Stem Cell Transplantation;
Stem Cells;
Triglycerides;
Tumor Necrosis Factor-alpha;
Vascular Cell Adhesion Molecule-1;
Vascular Endothelial Growth Factor A
- From:Yonsei Medical Journal
2019;60(11):1036-1044
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study aimed to investigate the effect of adipose-derived stem cell (ADSC) transplantation on atherosclerosis (AS) and its underlying mechanisms. MATERIALS AND METHODS: In our study, rat AS model was established, and ADSCs were isolated and cultured. Atherosclerotic plaque and pathological symptoms of thoracic aorta were measured by Oil Red O staining and Hematoxylin-Eosin staining, respectively. Total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were measured by an automatic biochemical analyzer. Expressions of vascular endothelial growth factor (VEGF), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), aortic endothelin-1 (ET-1), interleukin-6 (IL-6), c-reactive protein (CRP), and tumor necrosis factor α (TNF-α) were measured by enzyme linked immunosorbent assay, VEGF, VCAM-1, ICAM-1, ET-1, respectively, and NF-κB p65 mRNA expressions were detected by quantitative real-time polymerase chain reaction. Protein expressions of VEGF, VCAM-1, ICAM-1, ET-1, NF-κB p65, p-NF-κB p65, and IκBα were measured by western blot. Moreover, NF-κB p65 expression was measured by immunofluorescence staining. RESULTS: ADSC transplantation alleviated the pathological symptoms of aortic AS. ADSC transplantation decreased the levels of TC, TG, and LDL-C and increased serum HDL-C level. Meanwhile, ADSC transplantation decreased the levels of IL-6, CRP, and TNF-α in AS rats. Moreover, the expressions of VEGF, ET-1, VCAM-1, and ICAM-1 were decreased by ADSC transplantation. ADSC transplantation inhibited phosphorylation of NF-κB p65 and promoted IκBα expression in AS rats. CONCLUSION: Our study demonstrated that ADSC transplantation could inhibit vascular inflammatory responses and endothelial dysfunction by suppressing NF-κB pathway in AS rats.
