Preparation and Characterization of Human Adipose Tissue-Derived Extracellular Matrix, Growth Factors, and Stem Cells: A Concise Review
10.1007/s13770-019-00199-7
- Author:
So Young CHUN
1
;
Jeong Ok LIM
;
Eun Hye LEE
;
Man Hoon HAN
;
Yun Sok HA
;
Jun Nyung LEE
;
Bum Soo KIM
;
Min Jeong PARK
;
MyungGu YEO
;
Bongsu JUNG
;
Tae Gyun KWON
Author Information
1. BioMedical Research Institute, Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu 41940, Republic of Korea.
- Publication Type:Review
- Keywords:
Human adipose tissue;
Extracellular matrix;
Growth factors;
Adipose-derived stem cell;
Optimum method
- MeSH:
Adipose Tissue;
Collagen;
Elastin;
Extracellular Matrix;
Fibroblast Growth Factor 2;
Fibronectins;
Gene Expression;
Hepatocyte Growth Factor;
Humans;
Insulin;
Intercellular Signaling Peptides and Proteins;
Laminin;
Medical Waste;
Methods;
Nerve Growth Factor;
Platelet-Derived Growth Factor;
Stem Cells;
Transforming Growth Factor beta;
Vascular Endothelial Growth Factor A
- From:
Tissue Engineering and Regenerative Medicine
2019;16(4):385-393
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Human adipose tissue is routinely discarded as medical waste. However, this tissue may have valuable clinical applications since methods have been devised to effectively isolate adipose-derived extracellular matrix (ECM), growth factors (GFs), and stem cells. In this review, we analyze the literature that devised these methods and then suggest an optimal method based on their characterization results. METHODS: Methods that we analyze in this article include: extraction of adipose tissue, decellularization, confirmation of decellularization, identification of residual active ingredients (ECM, GFs, and cells), removal of immunogens, and comparing structural/physiological/biochemical characteristics of active ingredients. RESULTS: Human adipose ECMs are composed of collagen type I–VII, laminin, fibronectin, elastin, and glycosaminoglycan (GAG). GFs immobilized in GAG include basic fibroblast growth factor (bFGF), transforming growth factor beta 1(TGF-b1), insulin like growth factor 1 (IGF-1), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), BMP4 (bone morphogenetic protein 4), nerve growth factor (NGF), hepatocyte growth factor (HGF), and epithermal growth factor (EGF). Stem cells in the stromal-vascular fraction display mesenchymal markers, self-renewal gene expression, and multi-differentiation potential. CONCLUSION: Depending on the preparation method, the volume, biological activity, and physical properties of ECM, GFs, and adipose tissue-derived cells can vary. Thus, the optimal preparation method is dependent on the intended application of the adipose tissue-derived products.
