Preparation and Characterization of Nanocomposite Scaffolds (Collagen/β-TCP/SrO) for Bone Tissue Engineering
10.1007/s13770-019-00184-0
- Author:
Hamid GOODARZI
1
;
Sameereh HASHEMI-NAJAFABADI
;
Nafiseh BAHEIRAEI
;
Fatemeh BAGHERI
Author Information
1. Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Jalal ale Ahmad Highway, P.O. Box 14115-114, Tehran, Iran. s.hashemi@modares.ac.ir
- Publication Type:Original Article
- Keywords:
Collagen;
β-TCP;
SrO;
Freeze drying;
Bone tissue engineering
- MeSH:
Alkaline Phosphatase;
Animals;
Biopolymers;
Bone and Bones;
Bone Marrow;
Collagen;
Fourier Analysis;
Freeze Drying;
In Vitro Techniques;
Ions;
Mesenchymal Stromal Cells;
Nanocomposites;
Nanoparticles;
Porosity;
Rats;
Spectrum Analysis;
Strontium;
X-Ray Diffraction
- From:
Tissue Engineering and Regenerative Medicine
2019;16(3):237-251
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Nowadays, production of nanocomposite scaffolds based on natural biopolymer, bioceramic, and metal ions is a growing field of research due to the potential for bone tissue engineering applications. METHODS: In this study, a nanocomposite scaffold for bone tissue engineering was successfully prepared using collagen (COL), beta-tricalcium phosphate (β-TCP) and strontium oxide (SrO). A composition of β-TCP (4.9 g) was prepared by doping with SrO (0.05 g). Biocompatible porous nanocomposite scaffolds were prepared by freeze-drying in different formulations [COL, COL/β-TCP (1:2 w/w), and COL/β-TCP-Sr (1:2 w/w)] to be used as a provisional matrix or scaffold for bone tissue engineering. The nanoparticles were characterized by X-ray diffraction, Fourier transforms infrared spectroscopy and energy dispersive spectroscopy. Moreover, the prepared scaffolds were characterized by physicochemical properties, such as porosity, swelling ratio, biodegradation, mechanical properties, and biomineralization. RESULTS: All the scaffolds had a microporous structure with high porosity (~ 95–99%) and appropriate pore size (100–200 µm). COL/β-TCP-Sr scaffolds had the compressive modulus (213.44 ± 0.47 kPa) higher than that of COL/β-TCP (33.14 ± 1.77 kPa). In vitro cytocompatibility, cell attachment and alkaline phosphatase (ALP) activity studies performed using rat bone marrow mesenchymal stem cells. Addition of β-TCP-Sr to collagen scaffolds increased ALP activity by 1.33–1.79 and 2.92–4.57 folds after 7 and 14 days of culture, respectively. CONCLUSION: In summary, it was found that the incorporation of Sr into the collagen-β-TCP scaffolds has a great potential for bone tissue engineering applications.
