Proliferation Profile of Uterine Endometrial Stromal Cells during In Vitro Culture with Gonadotropins: Recombinant versus Urinary Follicle Stimulating Hormone
10.1007/s13770-018-0156-4
- Author:
Yong Jin KIM
1
;
Yoon Young KIM
;
Da Young SONG
;
Sang Hoon LEE
;
Chan Woo PARK
;
Hoon KIM
;
Seung Yup KU
Author Information
1. Department of Obstetrics and Gynecology, Korea University Medical Center, 73 Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Uterus;
Endometrial stromal cells;
In vitro culture
- MeSH:
Animals;
Bromodeoxyuridine;
Cell Cycle;
Chorionic Gonadotropin;
Cyclin D1;
Female;
Follicle Stimulating Hormone;
Gonadotropins;
Humans;
In Vitro Techniques;
Mice;
Stromal Cells;
Tissue Engineering;
Uterus
- From:
Tissue Engineering and Regenerative Medicine
2019;16(2):131-139
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Provision of optimal endometrial stromal cells is essential in uterine tissue engineering. Culture of these cells is significantly influenced by gonadotropin hormones. This investigation attempted to define the proliferation profiles of murine uterine endometrial stromal cells during in vitro culture with recombinant follicle stimulating hormone (rFSH), urinary follicle stimulating hormone (uFSH), and human chorionic gonadotropin (hCG). METHODS: Murine uterine endometrial stromal cells were collected from 8-week-old mice and cultured in vitro up to 72 h, with rFSH, uFSH, or hCG. Cell cycles were analyzed by BrdU assay, and cyclin D1 expression was evaluated according to dose and duration of gonadotropin treatment. RESULTS: BrdU assay showed a further inhibitory effect on murine uterine endometrial stromal cell proliferation when cultured with rFSH compared to uFSH, and a similar inhibitory proliferation profile when cultured with hCG at a specific range of concentrations. The expression of cyclin D1 of murine uterine endometrial stromal cells was down-regulated when cultured with rFSH, uFSH, or hCG, compared to control. CONCLUSIONS: FSH may inhibit the proliferation of murine uterine endometrial stromal cells during in vitro culture. rFSH may have more significant inhibitory effects on the proliferation of endometrial stromal cells than uFSH. Establishing an optimal endocrine milieu is necessary using more advanced combination of female hormones for in vitro culture of this type of cells.
