Conditioned Medium Enhances Osteogenic Differentiation of Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells
10.1007/s13770-018-0173-3
- Author:
Siying ZHONG
1
;
Xufeng HE
;
Yuexia LI
;
Xiangxin LOU
Author Information
1. Department of Bioengineering, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, China. xiangxin@dhu.edu.cn
- Publication Type:Original Article
- Keywords:
Induced pluripotent stem cells;
Mesenchymal stem cells;
Osteoblasts;
Conditioned medium;
Osteogenic differentiation
- MeSH:
Alkaline Phosphatase;
Animals;
Bone and Bones;
Culture Media, Conditioned;
Induced Pluripotent Stem Cells;
Mesenchymal Stromal Cells;
Mice;
Osteoblasts;
Phenotype
- From:
Tissue Engineering and Regenerative Medicine
2019;16(2):141-150
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Recent studies have shown that induced pluripotent stem cells (iPSCs) could be differentiated into mesenchymal stem cells (MSCs) with notable advantages over iPSCs per se. In order to promote the application of iPSC-MSCs for osteoregenerative medicine, the present study aimed to assess the ability of murine iPSC-MSCs to differentiate into osteoblast phenotype. METHODS: Osteogenic differentiation medium, blending mouse osteoblast-conditioned medium (CM) with basic medium (BM) at ratio 3:7, 5:5 and 7:3, were administered to iPSC-MSCs, respectively. After 14 days, differentiation was evaluated by lineage-specific morphology, histological stain, quantitative reverse transcription-polymerase chain reaction and immunostaining. RESULTS: The osteogenesis-related genes, alp, runx2, col1 and ocn expressions suggest that culture medium consisting of CM:BM at the ratio of 3:7 enhanced the osteogenic differentiation more than other concentrations that were tested. In addition, the alkaline phosphatase activity and osteogenic marker Runx2 expression demonstrate that the combination of CM and BM significantly enhanced the osteogenic differentiation of iPSC-MSCs. CONCLUSION: In summary, this study has shown that osteoblast-derived CM can dramatically enhance osteogenic differentiation of iPSC-MSCs toward osteoblasts. Results from this work will contribute to optimize the osteogenic induction conditions of iPSC-MSCs and will assist in the potential application of iPSC-MSCs for bone tissue engineering.
