D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation
10.1007/s13770-018-0171-5
- Author:
WeiKang ZHAO
1
;
Bin HE
;
Ao ZHOU
;
Yuling LI
;
Xiaojun CHEN
;
Qiming YANG
;
Beike CHEN
;
Bo QIAO
;
Dianming JIANG
Author Information
1. The First Affiliated Hospital of Chongqing Medical University, No 1 Medicine Road, Yuzhong District, Chongqing 400016, People's Republic of China. qiaobo1985@163.com, jdm571026@vip.163.com
- Publication Type:Original Article
- Keywords:
nHA/PA66/D-RADA16-RGD;
Bone defect;
Bone regeneration;
Peptide hydrogel
- MeSH:
Actin Cytoskeleton;
Blotting, Western;
Bone Regeneration;
Cell Count;
Cell Culture Techniques;
Circular Dichroism;
Endopeptidase K;
Fluorescent Antibody Technique;
In Vitro Techniques;
Microscopy, Electron, Scanning;
Microscopy, Electron, Transmission;
Osteogenesis;
Sincalide;
Spectrum Analysis
- From:
Tissue Engineering and Regenerative Medicine
2019;16(2):177-189
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) is a composite used widely in the repair of bone defects. However, this material is insufficient bioactivity. In contrast, D-RADA16-RGD self-assembling peptide (D-RADA16-RGD sequence containing all D-amino acids is Ac-RADARADARADARADARGDS-CONH2) shows admirable bioactivity for both cell culture and bone regeneration. Here, we describe the fabrication of a favorable biomaterial material (nHA/PA66/D-RADA16-RGD). METHODS: Proteinase K and circular dichroism spectroscopy were employed to test the stability and secondary structural properties of peptide D-RADA16-RGD respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the surface of these materials. Confocal laser scanning (CLS), cell counting kit-8 tests (CCK-8), alizarin red S staining, cell immunofluorescence analysis and Western blotting were involved in vitro. Also biosafety and bioactivity of them have been evaluated in vivo. RESULTS: Proteinase K and circular dichroism spectroscopy demonstrated that D-RADA16-RGD in nHA/PA66 was able to form stable-sheet secondary structure. SEM and TEM showed that the D-RADA16-RGD material was 7–33 nm in width and 130–600 nm in length, and the interwoven pore size ranged from 40 to 200 nm. CLS suggests that cells in nHA/PA66/D-RADA16-RGD group were linked to adjacent cells with more actin filaments. CCK-8 analysis showed that nHA/PA66/D-RADA16-RGD revealed good biocompatibility. The results of Alizarin-red S staining and Western blotting as well as vivo osteogenesis suggest nHA/PA66/D-RADA16-RGD exhibits better bioactivity. CONCLUSION: This study demonstrates that our nHA/PA66/D-RADA16-RGD composite exhibits reasonable mechanical properties, biocompatibility and bioactivity with promotion of bone formation.
