Long non-coding RNA T-cell leukemia/lymphoma 6 serves as a sponge for miR-21 modulating the cell proliferation of retinoblastoma through PTEN
10.4196/kjpp.2019.23.6.449
- Author:
Sisi TAO
1
;
Weidong WANG
;
Pengfei LIU
;
Hua WANG
;
Weirong CHEN
Author Information
1. Department of Science and Education, Changsha Hospital for Maternal & Child Health Care, Changsha 410007, Hunan, China. 411238793@qq.com, jiayinxy2006@126.com
- Publication Type:Original Article
- Keywords:
Cell proliferation;
miRNA 21;
Phosphate and tension homology deleted on chromsome ten (PTEN);
Retinoblastoma
- MeSH:
Apoptosis;
Cell Line;
Cell Proliferation;
MicroRNAs;
Porifera;
Repression, Psychology;
Retinoblastoma;
RNA, Long Noncoding;
RNA, Untranslated;
T-Lymphocytes
- From:The Korean Journal of Physiology and Pharmacology
2019;23(6):449-458
- CountryRepublic of Korea
- Language:English
-
Abstract:
Retinoblastoma (Rb) is one of the most common eye malignancies occur in childhood. The crucial roles of non-coding RNAs, particularly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been widely reported in Rb progression. In the present study, we found the expression of lncRNA T-cell leukemia/lymphoma 6 (TCL6) was significantly downregulated in Rb tissues and cell lines. Knockdown of lncRNA TCL6 promoted cell proliferation while reduced cell apoptosis in Rb cells. Moreover, lncRNA TCL6 serves as a sponge for miR-21, a previously-reported oncogenic miRNA in Rb, by direct targeting to negatively regulated miR-21 expression, therefore modulating Rb proliferation through miR-21. TCL6 overexpression inhibited Rb cell proliferation while miR-21 overexpression exerted an opposing effect; the effect of TCL6 overexpression was partially attenuated by miR-21 overexpression. PTEN/PI3K/AKT signaling pathway was involved in lncRNA TCL6/miR-21 axis modulating Rb cell proliferation. Taken together, lncRNA TCL6 serves as a tumor suppressor by acting as a sponge for miR-21 to counteract miR-21-mediated PTEN repression.
