Effects of rosmarinic acid on immunoregulatory activity and hepatocellular carcinoma cell apoptosis in H22 tumor-bearing mice
10.4196/kjpp.2019.23.6.501
- Author:
Wen CAO
1
;
Kai MO
;
Sijun WEI
;
Xiaobu LAN
;
Wenjuan ZHANG
;
Weizhe JIANG
Author Information
1. Department of Pharmacy, Guangxi International Zhuang Medicine Hospital, Guangxi 530200, China. mosquito1106@foxmail.com
- Publication Type:Original Article
- Keywords:
Apoptosis;
Hepatocellular carcinoma;
Immunoregulation;
Rosmarinic acid
- MeSH:
Animals;
Apoptosis;
Boraginaceae;
Carcinoma, Hepatocellular;
Caspase 3;
Cytokines;
Interleukin-10;
Interleukin-6;
Interleukins;
Lamiaceae;
Mice;
STAT3 Transcription Factor;
Therapeutic Uses;
Tumor Microenvironment
- From:The Korean Journal of Physiology and Pharmacology
2019;23(6):501-508
- CountryRepublic of Korea
- Language:English
-
Abstract:
Rosmarinic acid (RA) is a natural polyphenolic compound that exists in many medicinal species of Boraginaceae and Lamiaceae. The previous studies have revealed that RA had therapeutic effects on hepatocellular carcinoma (HCC) in the H22-xenograft models by inhibiting the inflammatory cytokines and NF-κB p65 pathway in the tumor microenvironment. However, its molecular mechanisms of immunoregulation and pro-apoptotic effect in HCC have not been fully explored. In the present study, RA at 75, 150, and 300 mg/kg was given to H22 tumor-bearing mice via gavage once a day for 10 days. The results showed that RA can effectively inhibit the tumor growth through regulating the ratio of CD4⁺/CD8⁺ and the secretion of interleukin (IL)-2 and interferon-γ, inhibiting the expressions of IL-6, IL-10 and signal transducer and activator of transcription 3, thereby up-regulating Bax and Caspase-3 and down-regulating Bcl-2. The underlying mechanisms involved regulation of immune response and induction of HCC cell apoptosis. These results may provide a more comprehensive perspective to clarify the anti-tumor mechanism of RA in HCC.
