Alteration of mitochondrial DNA content modulates antioxidant enzyme expressions and oxidative stress in myoblasts
10.4196/kjpp.2019.23.6.519
- Author:
Kyung Ho MIN
1
;
Wan LEE
Author Information
1. Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 38066, Korea. wanlee@dongguk.ac.kr
- Publication Type:Original Article
- Keywords:
Antioxidant;
Catalase;
Glutathione peroxidase;
Mitochondrial DNA;
Myoblasts;
Reactive oxygen species
- MeSH:
Catalase;
DNA, Mitochondrial;
Glutathione;
Glutathione Disulfide;
Glutathione Peroxidase;
Hand;
Homeostasis;
Myoblasts;
Oxidation-Reduction;
Oxidative Stress;
Reactive Oxygen Species
- From:The Korean Journal of Physiology and Pharmacology
2019;23(6):519-528
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mitochondrial dysfunction is closely associated with reactive oxygen species (ROS) generation and oxidative stress in cells. On the other hand, modulation of the cellular antioxidant defense system by changes in the mitochondrial DNA (mtDNA) content is largely unknown. To determine the relationship between the cellular mtDNA content and defense system against oxidative stress, this study examined a set of myoblasts containing a depleted or reverted mtDNA content. A change in the cellular mtDNA content modulated the expression of antioxidant enzymes in myoblasts. In particular, the expression and activity of glutathione peroxidase (GPx) and catalase were inversely correlated with the mtDNA content in myoblasts. The depletion of mtDNA decreased both the reduced glutathione (GSH) and oxidized glutathione (GSSG) slightly, whereas the cellular redox status, as assessed by the GSH/GSSG ratio, was similar to that of the control. Interestingly, the steady-state level of the intracellular ROS, which depends on the reciprocal actions between ROS generation and detoxification, was reduced significantly and the lethality induced by H₂O₂ was alleviated by mtDNA depletion in myoblasts. Therefore, these results suggest that the ROS homeostasis and antioxidant enzymes are modulated by the cellular mtDNA content and that the increased expression and activity of GPx and catalase through the depletion of mtDNA are closely associated with an alleviation of the oxidative stress in myoblasts.
