Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
10.4196/kjpp.2019.23.3.161
- Author:
Wan Guo YU
1
;
Yun HE
;
Yun Fang CHEN
;
Xiao Yao GAO
;
Wan E NING
;
Chun You LIU
;
Ting Fan TANG
;
Quan LIU
;
Xiao Cheng HUANG
Author Information
1. Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, China. yuwanguo@hotmail.com
- Publication Type:Original Article
- Keywords:
Adipogenesis;
Anti-obesity;
Fumigaclavine C;
Lipolysis
- MeSH:
Adipocytes;
Adipogenesis;
Animals;
Aspergillus;
Body Weight;
Carrier Proteins;
Diet, High-Fat;
Glycerol;
Injections, Intraperitoneal;
Intra-Abdominal Fat;
Lipase;
Lipolysis;
Lipoprotein Lipase;
Mice;
Mice, Obese;
Peroxisomes;
Rhizophoraceae;
Sterol Esterase;
Transcription Factors
- From:The Korean Journal of Physiology and Pharmacology
2019;23(3):161-169
- CountryRepublic of Korea
- Language:English
-
Abstract:
Fumigaclavine C (FC), an active indole alkaloid, is obtained from endophytic Aspergillus terreus (strain No. FC118) by the root of Rhizophora stylosa (Rhizophoraceae). This study is designed to evaluate whether FC has anti-adipogenic effects in 3T3-L1 adipocytes and whether it ameliorates lipid accumulation in high-fat diet (HFD)-induced obese mice. FC notably increased the levels of glycerol in the culture supernatants and markedly reduced lipid accumulation in 3T3-L1 adipocytes. FC differentially inhibited the expressions of adipogenesis-related genes, including the peroxisome proliferator-activated receptor proteins, CCAAT/enhancer-binding proteins, and sterol regulatory element-binding proteins. FC markedly reduced the expressions of lipid synthesis-related genes, such as the fatty acid binding protein, lipoprotein lipase, and fatty acid synthase. Furthermore, FC significantly increased the expressions of lipolysis-related genes, such as the hormone-sensitive lipase, Aquaporin-7, and adipose triglyceride lipase. In HFD-induced obese mice, intraperitoneal injections of FC decreased both the body weight and visceral adipose tissue weight. FC administration significantly reduced lipid accumulation. Moreover, FC could dose-dependently and differentially regulate the expressions of lipid metabolism-related transcription factors. All these data indicated that FC exhibited anti-obesity effects through modulating adipogenesis and lipolysis.
