Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
10.3347/kjp.2019.57.3.309
- Author:
Li HE
1
;
Zheng Ming FANG
;
Ting XUE
;
Er Fu ZHANG
;
Chun Li AN
Author Information
1. Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University, Shenyang 110122, PR. China. cmucl@126.com
- Publication Type:Brief Communication
- Keywords:
Spirometra erinaceieuropaei;
spargana;
cox1;
genetic analysis
- MeSH:
China;
DNA;
Genetic Variation;
Humans;
Polymerase Chain Reaction;
Sparganum;
Spirometra
- From:The Korean Journal of Parasitology
2019;57(3):309-312
- CountryRepublic of Korea
- Language:English
-
Abstract:
Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97–100% similarity to the reference sequence of S. erinaceieuropaei and 88–89% to the reference sequence of S. decipiens. There were 1–12 bases different between these worms, but no obvious genetic variation (0–3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1–3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.
