Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells
- Author:
Pu Reum SUN
1
;
Fei Fei GAO
;
Hei Gwon CHOI
;
Wei ZHOU
;
Jae Min YUK
;
Jaeyul KWON
;
Young Ha LEE
;
Guang Ho CHA
Author Information
- Publication Type:Original Article
- Keywords: T. gondii; reactive oxygen species; diphenyleneiodonium; N-acetyl-L-cysteine; ARPE-19 cell
- MeSH: Acetylcysteine; Ascorbic Acid; Humans; Mitochondria; Parasites; Reactive Oxygen Species; Retinaldehyde; Superoxides; Toxoplasma; Toxoplasmosis
- From:The Korean Journal of Parasitology 2019;57(2):83-92
- CountryRepublic of Korea
- Language:English
- Abstract: Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.
