Screening of Sera from Patients with Pancreatitis by an Apoptosis Assay of Skin-derived Cells
10.4166/kjg.2019.74.4.219
- Author:
Ae Eun SEOK
1
;
Byoung Kwan SON
;
Jiyeong LEE
;
Kwang Hyun CHUNG
;
You Rim LEE
;
Doojin KIM
;
Byung Heun CHA
;
Hee Gyoo KANG
Author Information
1. Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, Daejeon, Korea. kanghg@eulji.ac.kr
- Publication Type:Original Article
- Keywords:
Acute pancreatitis;
Skin tissue-derived cell line;
Apoptosis;
Alcohol-induced pancreatitis
- MeSH:
Apoptosis;
Cell Line;
Flow Cytometry;
Gallstones;
Healthy Volunteers;
Humans;
Mass Screening;
Mortality;
Pancreatitis;
Pancreatitis, Alcoholic;
Skin
- From:The Korean Journal of Gastroenterology
2019;74(4):219-226
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: An excessive inflammatory response is typical in acute pancreatitis and a significant cause of early mortality in severe acute pancreatitis. This is believed to be caused by inflammatory molecules or upregulated cytokine levels in the serum of patients. The aim of this study was to identify the serum-mediated apoptosis-inducing effects in acute pancreatitis patients. METHODS: A skin tissue-derived cell line, BJ, was treated for 24 hours with the sera of 22 healthy volunteers (control) and 71 acute pancreatitis patients (22 with gallstone pancreatitis, 16 with alcoholic pancreatitis, and 11 with pancreatitis with other causes) collected at the time of hospital admission (active) and discharge (resolved). Apoptosis was analyzed by flow cytometry. RESULTS: The average percentage of living cells, early apoptotic cells, and late apoptotic cells ranged from 78.8% to 85.0%, 5.5% to 7.3%, and 7.7% to 13.1%, respectively. The number of live cells increased significantly using the serum from the resolved state of gallstone-induced pancreatitis. In addition, the number of early apoptotic cells increased significantly using the serum from the resolved state of pancreatitis with other causes. The number of late apoptotic cells decreased significantly with the serum from the resolved state compared to the active state of gallstone- and alcohol-induced pancreatitis. CONCLUSIONS: Serum samples from patients with pancreatitis induced a change in the apoptosis profiles of skin-derived cells. These results indicate changes in the serum components in patients with acute pancreatitis.
