Expression of Metabotropic Glutamate Receptors in the Medial Vestibular Nucleus Following Acute Hypotension in Rats
- Author:
Myoung Ae CHOI
1
;
Nari KIM
;
Sang Eon PARK
;
Byung Geon PARK
;
Min Sun KIM
;
Byung Rim PARK
Author Information
1. Department of Physiology, Wonkwang University School of Medicine, and Brain Science Institute at Wonkwang University, Iksan, Korea. byungp@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Metabotropic glutamate receptor;
Ionotropic glutamate receptor;
Homer1a;
Medial vestibular nucleus;
Acute hypotension
- MeSH:
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;
Animals;
Blotting, Western;
Glutamic Acid;
Hypotension;
N-Methylaspartate;
Neurotransmitter Agents;
Phosphotransferases;
Rats;
Receptors, Metabotropic Glutamate;
Receptors, N-Methyl-D-Aspartate;
Vestibular Nuclei
- From:Journal of the Korean Balance Society
2012;11(4):131-137
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Acute hypotension induces expression of c-Fos protein and phosphorylated extracellular signal-regulated kinase (pERK), and glutamate release in the vestibular nuclei. Expression of c-Fos protein and pERK is mediated by the excitatory neurotransmitter, glutamate. In this study, the signaling pathway of glutamate in the vestibular nuclei following acute hypotension was investigated. MATERIALS AND METHODS: Expression of metabotropic glutamate receptors (mGluRs) was measured by Western blotting in the medial vestibular nucleus following acute hypotension in rats. RESULTS: Expression of pGluR1 Ser831, a subtype of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, peaked at 30 minutes after acute hypotension insult, and expression of pNR2B, a subtype of N-methyl-D-aspartate (NMDA) receptors, peaked at 2 hours after acute hypotension insult. Acute hypotension induced expression of Homer1a and group I mGluR in the medial vestibular nucleus. Expression of mGluR1 and mGluR5 peaked at 6 hours following acute hypotension insults. CONCLUSION: These results suggest that afferent signals from the peripheral vestibular receptors, resulting from acute hypotension insult, are transmitted through group I mGluRs as well as AMPA and NMDA receptors in the vestibular system.
