The Relationship between Wnt Antagonist Genes Polymorphisms and Changes in Production of Osteoprotegerin and Soluble Receptor Activator of NF-kB by Whole Blood Cells after Hormone Therapy
- Author:
Kyung Eui PARK
1
;
Dong Ock LEE
;
Hoon KIM
;
Seung Yup KU
;
Seok Hyun KIM
;
Young Min CHOI
;
Jung Gu KIM
Author Information
1. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. kimjg@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Hormone therapy;
Osteoprotegerin;
Receptor activator of nuclear factor-kB ligand;
Whole blood cell;
Wnt antagonist polymorphism
- MeSH:
Blood Cells;
Female;
Genotype;
Humans;
NF-kappa B;
Osteoprotegerin;
Polymorphism, Single Nucleotide;
Receptor Activator of Nuclear Factor-kappa B;
Signal Transduction
- From:Journal of Korean Society of Osteoporosis
2012;10(3):112-118
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To investigate the relationship between single nucleotide polymorphism (SNP)s in Wnt antagonist genes, and production of osteoprotegerin (OPG) and soluble receptor activator of NF-kappaB ligand (sRANKL) by whole blood cells after hormone therapy (HT) in postmenopausal Korean women. MATERIALS AND METHODS: The Dkk1 c.318A>G, Dkk2 c.437G>A, Dkk3 c.1003A>G polymorphisms and sFRP3 c.970C>G, sFRP4 c.958C>A, and c.1019G>A polymorphisms, and sFRP5 c.20G>C polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), direct sequencing, and Taqman assay in 75 postmenopausal Korean women receiving estrogen-progestogen therapy. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells (WBC) before and after HT of 6 months were also measured. RESULTS: Changes in the production of OPG and sRANKL by lipopolysaccharide-stimulated WBC, and in ratios of sRANKL(x1,000)/OPG after HT of 6 months were not different according to SNPs in Wnt signal pathway genes except Dkk1 c.318A>G SNP. The AA genotype of Dkk1 c.318A>G SNP showed significantly higher changes (p<0.05) in ratios of sRANKL(x1,000)/OPG compared to other genotypes. There were no significant differences in changes in the production of OPG and sRANKL, and in ratios of sRANKL(x1,000)/OPG among combined genotypes of sFRP4 c.958C>A, and c.1019G>A polymorphisms after HT. CONCLUSIONS: Dkk1 c.318A>G SNP are related with changes in ratios of sRANKL(x1,000)/OPG in terms of the production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells after HT.
