The Relationship between Polymorphisms in Tumor Necrosis Factor (TNF) Genes and Changes in Bone Mineral Density after Hormone Therapy in Postmenopausal Korean Women
- Author:
Sungwook CHUN
1
;
Jung Gu KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Inje University, Korea.
- Publication Type:Original Article
- Keywords:
Korean postmenopausal women;
TNF;
Gene polymorphism;
Hormone therapy;
BMD
- MeSH:
Absorptiometry, Photon;
Alkaline Phosphatase;
Bone Density;
Calcium;
Collagen Type I;
Enzyme-Linked Immunosorbent Assay;
Female;
Femur Neck;
Humans;
Immunoassay;
Lymphotoxin-alpha;
Osteocalcin;
Parathyroid Hormone;
Peptides;
Phosphorus;
Spine;
Tumor Necrosis Factor-alpha
- From:Journal of Korean Society of Osteoporosis
2011;9(1):66-79
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To determine the association between polymorphisms in tumor necrosis factor-alpha (TNF-alpha), and TNF-beta genes, and changes in bone mineral density (BMD) after hormone therapy (HT) in postmenopausal Korean women. MATERIALS & METHODS: The TNF-alpha G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-beta A252G polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or Taqman assay in 218 postmenopausal Korean women receiving sequential HT for 1 year. Bone mineral density (BMD) at the lumbar spine and femoral neck was examined by dual energy X-ray absorptiometry. Serum levels of bone specific alkaline phosphatase (BAP), osteocalcin (OST), type I C-telopeptide breakdown products (CTX), parathyroid hormone (PTH), calcium, and phosphorus were measured using enzyme-linked immunosorbent assay (ELISA), immunoassay, and atomic absorptiometry, respectively. RESULTS: The TNF-alpha G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-beta A252G polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or Taqman assay in 218 postmenopausal Korean women receiving sequential HT for 1 year. Bone mineral density (BMD) at the lumbar spine and femoral neck was examined by dual energy X-ray absorptiometry. Serum levels of bone specific alkaline phosphatase (BAP), osteocalcin (OST), type I C-telopeptide breakdown products (CTX), parathyroid hormone (PTH), calcium, and phosphorus were measured using enzyme-linked immunosorbent assay (ELISA), immunoassay, and atomic absorptiometry, respectively CONCLUSIONS: The TNF-alpha G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-beta A252G polymorphisms are not associated with changes in BMD after HT in postmenopausal Korean women.
