Anti-inflammatory effect of aged black garlic on 12-O-tetradecanoylphorbol-13-acetate-induced dermatitis in mice
10.4162/nrp.2019.13.3.189
- Author:
Bo Ram YOU
1
;
Jae Myung YOO
;
Seong Yeon BAEK
;
Mee Ree KIM
Author Information
1. Department of Food and Nutrition, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea. mrkim@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Contact dermatitis;
COX-2;
cytokines;
macrophage;
NF-kappa B
- MeSH:
Animals;
Cyclooxygenase 2;
Cytokines;
Cytosol;
Dermatitis;
Dermatitis, Contact;
Dinoprostone;
Ear;
Edema;
Garlic;
In Vitro Techniques;
Inhibitory Concentration 50;
Interleukin-6;
Macrophages;
Mice;
Necrosis;
Neuroprotection;
NF-kappa B;
Nitric Oxide Synthase Type II
- From:Nutrition Research and Practice
2019;13(3):189-195
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: Although aged black garlic has various biological activities such as anti-allergy, anti-inflammation and neuroprotection, effect of aged black garlic on chemically contact dermatitis is unclarified. MATERIALS/METHODS: To evaluate anti-dermatitic activity of aged black garlic extract, we investigated effects of a fraction of aged black garlic extract (BG10) on both in vivo and in vitro. RESULTS: BG10 almost inhibited formation of nitric monoxide and interleukin-6 (IL-6; IC50, 7.07 µg/mL) at 25 µg/mL, and dose-dependently reduced production of tumor necrosis factor-α (TNF-α; IC50, 52.07 µg/mL) and prostaglandin E2 (IC50, 38.46 µg/mL) in lipopolysaccharide-stimulated RAW264.7 cells. In addition, BG10 significantly inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2 and nuclear NF-κB, and improved that of cytosolic levels of NF-κB and IκBα in the cells. Consistent with in vitro studies, BG10 (0.5 mg/mL) not only reduced ear edema but also suppressed the formation of IL-6 and TNF-α induced by 12-O-tetradecanoylphorbol-13-acetate in ear tissues of mice. CONCLUSIONS: These findings suggest BG10 has anti-dermatitic activity through inhibiting activation of macrophages. Therefore, such effects of BG10 may provide information for the application of aged black garlic for prevention and therapy of contact dermatitis.
